超表达杨树RPEase基因促进了拟南芥的生长发育

发布时间：2018-05-01 12:24

  本文选题：杨树 + 卡尔文循环　； 参考：《北京林业大学学报》2016年05期

【摘要】：卡尔文循环是光合作用CO_2同化的重要途径,在植物生长发育过程中起着重要作用。磷酸核酮糖3-差向异构酶(RPEase:EC 5.1.3.1)是卡尔文循环再生阶段的一种重要酶类。本文从速生欧美杂交黑杨NE-19中克隆得到RPEase基因,构建植物表达载体,利用农杆菌花序侵染法转化野生型和突变体拟南芥,通过普通PCR检测和绿色荧光蛋白(GFP)观测进一步鉴定得到Ca MV35S:PdRPE:GFP超表达株系,然后对野生型、超表达株系、突变体和回补株系的生理指标进行测定。结果显示,超表达株系RPEase活性显著升高(P0.05)。在正常浇水的生长条件下,超表达株系相比于其他3个株系(野生型、突变体、回补株系),气孔数目减少,气孔变大,提高了植物的水分利用效率以及净光合速率,使得超表达株系有更好的生长优势,积累更多的淀粉。在10 d的短期干旱条件下,超表达株系的净光合速率和水分利用效率依然显著高于其他3个株系(P0.01)。因此,研究表明超表达RPEase基因会提高植物生物量的积累以及对短期干旱的抵抗能力。
[Abstract]:Calvin cycle is an important pathway of photosynthesis CO_2 assimilation and plays an important role in plant growth and development. RPEase: EC 5.1.3.1) is an important enzyme in Calvin cycle regeneration. In this paper, the RPEase gene was cloned from NE-19 of American and European hybrid, and the plant expression vector was constructed. The wild type and mutant Arabidopsis thaliana were transformed by Agrobacterium tumefaciens inflorescence infection. The Ca MV35S:PdRPE:GFP overexpression lines were further identified by PCR detection and GFP observation, and the physiological indexes of wild type, overexpression lines, mutants and complementary lines were determined. The results showed that the RPEase activity of the overexpressed lines increased significantly (P 0.05). Compared with other three lines (wild type, mutants, compensatory lines), the number of stomata decreased and stomata became larger, which increased the water use efficiency (Wue) and net photosynthetic rate (PN) of the plants. So that the super-expression lines have better growth advantage and accumulate more starch. Under the condition of short-term drought for 10 days, the net photosynthetic rate and water use efficiency of the overexpressed lines were still significantly higher than those of the other three lines. Therefore, overexpression of RPEase gene can increase plant biomass accumulation and resistance to short-term drought.
【作者单位】： 北京林业大学林木育种国家工程实验室;
【基金】：“十二五”国家科技支撑计划课题(2015BAD07B01) 国家自然科学基金项目(31270656) 北京市科学研究与研究生培养共建项目
【分类号】：S718.46
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本文编号：1829355