SCN5A基因H558R位点多态性与新疆地区维吾尔族，汉族心房颤动患者的相关性研究

发布时间：2018-05-01 15:38

本文选题：心房颤动 + SCN5A　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:心房颤动(房颤)是临床上常见的房性心律失常,近几年,SCN5A基因与房颤的关系受到高度关注,本文主要探讨新疆地区维吾尔族,汉族心房颤动(atrial fibrillation AF)患者与SCN5A基因H558R位点多态性的关系。方法:以维吾尔族,汉族心房颤动患者各100例作为病例组,以维吾尔族,汉族非心房颤动患者各100例作为对照组,收集标本,提取外周血DNA,运用聚合酶链反应(PCR)及DNA测序技术检测SCN5A基因H558R位点基因型及等位基因频率。结果:维吾尔族房颤组基因型及等位基因的分布与非房颤组之间比较有显著差异(P小于0.05),汉族房颤组基因型及等位基因的分布与非房颤组之间比较有显著差异(P小于0.05),维吾尔族房颤患者与汉族房颤患者SCN5A基因H558R位点基因型频率和等位基因频率之间存在差异(P0.05),logistic回归分析结果显示G等位基因可能是房颤的一个易患基因(OR=2.189,95%CI=1.360-3.523,P=0.001)。结论:新疆地区维吾尔族,汉族心房颤动的发生可能与SCN5A基因H558R位点多态性相关,G等位基因可能是心房颤动遗传易感性的独立危险因子。SCN5A基因H558R位点多态性在汉族与维吾尔族房颤患者分布中存在民族差异,可能与各民族不同的遗传因素,区域,生活习惯等有关。
[Abstract]:Objective: atrial fibrillation (AF) is a common atrial arrhythmia. In recent years, the relationship between SCN5A gene and atrial fibrillation is highly concerned. Relationship between polymorphism of H558R locus of SCN5A gene in patients with atrial fibrillation in Han nationality. Methods: 100 patients with atrial fibrillation in Uygur nationality and 100 patients with atrial fibrillation in Han nationality were selected as case group, 100 patients in Uygur nationality and 100 patients in Han nationality as control group. Polymerase chain reaction (PCR) and DNA sequencing were used to detect the genotype and allele frequency of H558R locus of SCN5A gene. Results: the distribution of genotypes and alleles in Uygur patients with atrial fibrillation was significantly different from that in non-atrial fibrillation patients (P < 0.05), and the distribution of genotypes and alleles in Han patients with atrial fibrillation was significantly different from that of non-atrial fibrillation patients (P < 0.05). The results of logistic regression analysis showed that G allele might be a susceptible gene of atrial fibrillation in Uygur patients with atrial fibrillation and Han nationality patients with atrial fibrillation. The results of logistic regression analysis showed that G allele might be a susceptible gene of atrial fibrillation, which was 1.360-3.523P0.001. Conclusion: Uygur people in Xinjiang, The occurrence of atrial fibrillation in Han nationality may be associated with the polymorphism of H558R locus of SCN5A gene. The allele G may be an independent risk factor of genetic susceptibility to atrial fibrillation .H558R locus polymorphism of SCN5A gene is distributed in patients with atrial fibrillation in Han and Uygur nationality. There are ethnic differences in China, May be related to different ethnic genetic factors, regions, habits and so on.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.75

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