CDX2基因干扰对人结肠癌细胞在裸鼠体内生长的影响

发布时间：2018-05-01 16:17

本文选题：结肠肿瘤 + 基因　；参考：《中国普通外科杂志》2017年04期

【摘要】：目的:探讨CDX2基因表达对结肠癌细胞生长的影响。方法:将体外转染CDX2-shRNA慢病毒、空慢病毒载体及无转染的人结肠癌细胞系SW480和HT29接种到裸鼠皮下,制备皮下移植瘤模型,观察各组移植瘤的生长情况,28d后处死裸鼠,剥取移植瘤称重,并分别用免疫组化、Westernblot检测各组移植瘤组织Ki-67与CDX2的表达。结果:与各自空白对照组(无转染的SW480或HT29细胞移植)比较,两个干扰组(转染CDX2-shRNA慢病毒的SW480或HT29细胞移植)移植瘤生长速度均明显加快、移植瘤质量明显增加(SW480:679.11mgvs.379.36mg;HT29:715.78mgvs.427.07mg),瘤组织Ki-67蛋白表达水平明显升高、CDX2蛋白表达明显下调(均P0.05)。两个阴性对照组(转染空慢病毒载体的SW480或HT29细胞移植)的各指标与各自空白对照组间均无明显差异(均P0.05)。结论:抑制CDX2基因的表达能促进人结肠癌细胞的裸鼠体内的生长,故CDX2可能是一种重要的抑癌基因。
[Abstract]:Objective: to investigate the effect of CDX2 gene expression on the growth of colon cancer cells. Methods: CDX2-shRNA lentivirus, empty lentivirus vector and human colon cancer cell lines SW480 and HT29 were inoculated into nude mice subcutaneously. The growth of transplanted tumor was observed for 28 days and the nude mice were killed. The expression of Ki-67 and CDX2 were detected by immunohistochemistry and Western blot. Results: compared with the control group (SW480 or HT29 cell transplantation without transfection), the tumor growth rate of the two interference groups (SW480 or HT29 cells transfected with CDX2-shRNA lentivirus) increased significantly. The quality of transplanted tumor was significantly increased. The expression of CDX2 protein was decreased significantly (all P 0.05), while the expression of Ki-67 protein in tumor tissue was significantly increased (P < 0.05), and the expression of Ki-67 protein in tumor tissue was significantly decreased (all P < 0.05), while HT29: 715.78 mgvs.427.07 mg / g of HT29: 715.78mgvs.427.07 mg / g respectively. There was no significant difference between the two negative control groups (SW480 or HT29 cells transfected with empty lentivirus vector) and their blank control groups (P 0.05). Conclusion: inhibiting the expression of CDX2 gene can promote the growth of human colon cancer cells in nude mice, so CDX2 may be an important tumor suppressor gene.
【作者单位】：西安交通大学第一附属医院普通外科;
【基金】：国家自然科学基金资助项目(81101874;81172362) 陕西省科学技术研究发展计划资助项目(2016SF-015;2015SF053) 陕西省科技统筹创新工程计划资助项目(2013KTCQ03-08)
【分类号】：R735.35

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