基于线粒体COI和Cytb基因的中国灰蝶三亚科主要类群间的系统发育关系分析

发布时间：2018-05-02 05:16

本文选题：鳞翅目 + 灰蝶科　；参考：《昆虫学报》2016年01期

【摘要】：【目的】针对中国灰蝶科中亲缘关系较近的3个主要亚科[灰蝶亚科(Lycaeninae)、线灰蝶亚科(Theclinae)以及眼灰蝶亚科(Polyommatina)],基于线粒体基因序列数据研究它们主要类群间的系统发育关系。【方法】对3亚科共53种灰蝶的线粒体COI和Cytb基因进行序列测定和序列变异分析,同时,基于最大似然法(maximum likelihood,ML)和贝叶斯法(bayesian inference,BI)等建树方法重建53种灰蝶的系统发育树。【结果】串联的2个基因共1 431 bp,其中保守位点855个,可变位点576个,简约信息位点488个;A+T的平均含量为74.5%,明显高于G+C的平均含量(25.5%)。系统树显示,灰蝶亚科以及眼灰蝶亚科均是单系发生,线灰蝶亚科则为并系群。全部灰蝶物种共分为三大支系:灰蝶亚科为第1支系;眼灰蝶亚科与线灰蝶亚科中的旖灰蝶族(Hypolycaenini)、富丽灰蝶族(Aphnaeini)分别构成单系群并互为姊妹群,它们共同构成第2支系;线灰蝶亚科中的美灰蝶族(Eumaeini)、玳灰蝶族(Deudorigini)、娆灰蝶族(Arhopalini)和线灰蝶族(Theclini)构成第3支系,其亲缘关系为:(((线灰蝶族+娆灰蝶族)+玳灰蝶族)+美灰蝶族)。【结论】本研究涉及的3个灰蝶亚科中,灰蝶亚科是一个独立的支系,眼灰蝶亚科与线灰蝶亚科之间有较近的亲缘关系,但它们内部主要类群间的系统发育关系还需要进一步的研究。
[Abstract]:[objective] to study the phylogeny of three major subfamilies (Lycaeninaeae, Theclinaeae) and Polyommatinia (Polyommatina), which are closely related to the family Cynidae in China, and to study their phylogeny on the basis of mitochondrial gene sequence data. [methods] Mitochondrial COI and Cytb genes of 53 species in 3 subfamilies were sequenced and sequenced. At the same time, the phylogenetic tree of 53 species of grey butterflies was reconstructed based on maximum likelihood method (MLL) and Bayesian inferenceBI (Bayesian method). [results] two genes in tandem were 1 431 BP, including 855 conserved loci and 576 variable loci. The average content of reduced information sites at 488 sites was 74.5, which was significantly higher than that of GC. The phylogenetic tree showed that both the subfamily and the subfamily Erythroidae were monophylogenetic, while the subfamily of Cynopteridae was a union group. All the species were divided into three branches: the first branch of the subfamily, the second branch of Hypolycaeniniidae, the Aphnaeinii, which formed a monophyletic group, and the second branch of Aphnaeiniae, respectively, and the second branch of Hypolycaeniniidae and Hypolycaeniniidae. Eumaeiniae, Deudoriginius, Arhopalinii and Theclinii from the subfamily Eumaeiniidae form the third branch. The phylogenetic relationship of the three subfamilies in this study was that of the three subfamilies in this study, one is an independent branch, and the other is the family of Dailai, which is a member of the Clariidae. [conclusion] among the three subfamilies in this study, the subfamily is an independent branch. There is a close relationship between the subfamilies of the Orydidae and the subfamilies, but the phylogenetic relationship among the main groups needs further study.
【作者单位】：安徽师范大学生命科学学院分子进化与生物多样性研究室;
【基金】：国家自然科学基金项目(41172004)
【分类号】：Q969

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