水稻窄卷叶突变体nrl7的鉴定与基因定位

发布时间：2018-05-02 05:17

本文选题：水稻 + 窄卷叶突变体　；参考：《植物学报》2016年03期

【摘要】：叶片的形态是理想株型的重要性状,叶片适度卷曲能提高水稻(Oryza sativa)群体的光能利用率,研究控制水稻叶片形态的相关基因能够进一步丰富株型理论。该研究在粳稻品系C275的群体中发现了1株自然变异的窄卷叶突变体nrl7(narrow rolled leaf 7)。与野生型相比,突变体的叶片变窄且向内卷曲;该突变体叶片连接中脉的泡状细胞严重变形,中脉与小叶脉之间的维管束数量均减少至1个。此外,突变体nrl7的株高、实粒数和实粒重均降低或减少,分别为野生型的88.46%、69.77%和68.98%,差异达极显著水平。叶片卷曲导致单叶光合速率减弱,与野生型相比,突变体的光合速率降低了17%,达极显著水平。突变体nr I7叶片的气孔导度、胞间CO2浓度和蒸腾速率则与野生型相比无明显变化。利用图位克隆的方法将目的基因定位于水稻第3染色体短臂上的分子标记RM5444和MM1300之间,物理距离约为185.14 kb。研究结果为该基因的克隆和进一步的功能分析奠定了基础。
[Abstract]:Leaf morphology is an important trait of ideal plant type. Leaf appropriate curling can improve the light energy utilization of rice (Oryza sativa) population. The study of related genes controlling leaf morphology of rice can further enrich the theory of plant type. In this study, 1 naturally variant narrow coiled leaf mutant nrl7 (narrow roll) was found in the population of the japonica rice line C275. Ed leaf 7). Compared with the wild type, the mutant leaves are narrower and inward curling; the mutant leaves are severely deformed in the middle vein, and the number of vascular bundles between the midvein and the small vein is reduced to 1. In addition, the height of the mutant nrl7, the real grain number and the real weight of the mutant, are reduced or reduced, respectively, 88.46%, 69.77% and 68.98 of the wild type. The leaf curl resulted in the decrease of photosynthetic rate of single leaf. Compared with the wild type, the photosynthetic rate of the mutant decreased by 17%, reaching a very significant level. The stomatal conductance of the mutant NR I7 leaves, the intercellular CO2 concentration and the transpiration rate were no obvious changes compared with the wild type. The target gene was located by the method of map cloning to locate the target gene. The molecular markers RM5444 and MM1300 on the short arm of the third chromosome of rice, and the physical distance of about 185.14 kb., lay the foundation for the cloning and further functional analysis of the gene.

【作者单位】：沈阳农业大学水稻研究所东北粳稻遗传改良与优质高效生产协同创新中心;
【基金】：教育部长江学者和创新团队发展计划(No.IRT13079) 国家自然科学基金(No.31201140)
【分类号】：S511

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