白菜谷胱甘肽过氧化物酶基因GPX的鉴定与分析

发布时间：2018-05-02 05:30

本文选题：白菜 + GPX基因　；参考：《浙江农业学报》2016年01期

【摘要】：为研究白菜谷胱甘肽过氧化物酶(Glutathione peroxidase,GPX)基因的生物学特征,从白菜全基因组数据库中筛选了10个GPX基因。基于公开的白菜转录组测序数据,分析了GPX基因在胚胎发育和器官中的表达情况,并且通过生物信息学手段对这10个GPX基因进行基因定位、启动子分析、基因结构、系统树构建和基因表达等研究。结果表明,Br GPX5基因的编码区中有6个内含子,其余基因均只有5个内含子,说明Br GPX5基因在进化过程中获得了一个内含子,导致氨基酸分子量增加;另外,每个GPX基因的启动子区都有2个及以上的E-box元件。
[Abstract]:In order to study the biological characteristics of glutathione peroxidase (Glutathione peroxidase) gene in Chinese cabbage, 10 GPX genes were screened from the whole genome database of Chinese cabbage. Based on the published transcriptome sequencing data of Chinese cabbage, the expression of GPX gene in embryonic development and organs was analyzed, and the gene location, promoter analysis and gene structure of the 10 GPX genes were analyzed by bioinformatics. Systematic tree construction and gene expression. The results showed that there were 6 introns in the coding region of the Br GPX5 gene and only 5 introns in the other genes, indicating that one intron was obtained in the evolution of the Br GPX5 gene, which resulted in the increase of the molecular weight of amino acids. Each promoter of GPX gene has two or more E-box elements.
【作者单位】：浙江省农业科学院蔬菜研究所;南京农业大学园艺学院;
【基金】：国家自然科学基金(31372058,31301788) 浙江省重大科技专项(2012C12903-6-2,2012C12903-3-7)
【分类号】：S634

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