强光照致TgAPPswePS1转基因鼠视网膜功能障碍和结构损伤

发布时间：2018-05-02 09:19

本文选题：光损伤 + 视网膜电生理　；参考：《眼科新进展》2017年04期

【摘要】：目的初步研究在光照损伤作用下TgAPPswePS1转基因鼠视网膜结构和功能的改变情况。方法给予6月龄阿尔茨海默病转基因鼠(TgAPPswePS1鼠)10 000 lux光照处理,每天光照12 h,连续光照6个月,光照处理为实验组,设鼠龄匹配的转基因鼠为对照组,在光照6个月后(即小鼠12个月时)对实验组与对照组小鼠进行视网膜电图检测,取材并进行切片,行HE染色并观察视网膜各层结构改变,予以视网膜厚度测量并进行统计学分析。结果对照组Rod反应a、b波波幅分别为(18.33±3.53)μV、(107.58±14.72)μV,Max反应a、b波波幅分别为(64.80±7.57)μV、(178.76±14.47)μV;实验组光照6个月后小鼠视网膜电图Rod反应a、b波波幅分别为(17.92±4.89)μV、(21.83±5.51)μV,Max反应的a、b波波幅分别为(18.23±4.44)μV、(24.50±4.49)μV;与对照组比较,Rod反应的b波和Max反应的a、b波的波幅下降明显,差异均有统计学意义(均为P0.05);在Rod和Max反应里a波和b波的潜伏期改变不明显。与对照组比较,经过光照处理后,实验组小鼠视网膜结构发生明显改变,以外核层与感光细胞外节明显,视网膜厚度明显变薄,为(102.34±9.38)μm,与对照组(181.32±13.47)μm比较差异有统计学意义(P=0.017)。结论高强度光照可以造成TgAPPswePS1鼠视网膜结构损伤和功能障碍。
[Abstract]:Objective to study the changes of retina structure and function in TgAPPswePS1 transgenic mice induced by light damage. Methods A 6-month-old transgenic mouse with Alzheimer's disease was treated with 10 000 lux light for 12 hours a day for 6 months. The experimental group was treated with light for 6 months, and the control group was used as the control group. After 6 months of illumination (12 months in mice), the electroretinogram of the experimental group and the control group were examined, the samples were taken and sliced, and the changes of the structure of each layer of the retina were observed by HE staining. Retinal thickness was measured and statistically analyzed. Results the amplitudes of Rod response to Rod were 107.58 卤14.72 渭 V, 178.76 卤14.47 渭 V and 18.92 卤4.89 卤5.83 卤5.51 渭 V, respectively, and 18.23 卤4.44 渭 V 卤4.49 渭 V, respectively, compared with those of the control group after 6 months of light exposure in the experimental group, which were 18.23 卤4.44 渭 V and 24.50 卤4.49 渭 V, respectively, in the experimental group after 6 months of illumination, the amplitudes of the Rod response of the experimental group were 17.92 卤4.89 渭 V and 21.83 卤5.51 渭 V ~ (-1) 渭 V ~ (-1) 渭 V ~ (-1), respectively, and those of the experimental group were 18.23 卤4.44) 渭 V ~ (24. 50) 渭 V ~ (4. 49) 渭 V, respectively. The amplitudes of B wave in Rod reaction and Ab wave in Max reaction decreased obviously. The difference was statistically significant (P 0.05), but there was no significant change in the latency of a wave and b wave in Rod and Max responses. Compared with the control group, the retinal structure of the experimental group was obviously changed, the outer nuclear layer and the photosensitive cell outer ganglia were obviously changed, and the retinal thickness was obviously thinned (102.34 卤9.38) 渭 m, which was significantly different from that of the control group (181.32 卤13.47) 渭 m. Conclusion High intensity illumination can cause retinal structure damage and dysfunction in TgAPPswePS1 rats.
【作者单位】：温州医科大学附属第二医院&育英儿童医院眼科;西安市第四医院眼科;广东医科大学衰老研究所东莞校区;中山大学中山眼科中心;
【基金】：国家自然科学基金资助(编号:81400424) 陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04) 温州市科技计划项目(编号:Y20140140)~~
【分类号】：R774.1

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