尼罗罗非鱼NITR基因的克隆鉴定与组织表达分析

发布时间：2018-05-02 16:18

本文选题：尼罗罗非鱼 + NITR蛋白　；参考：《生物技术通报》2017年05期

【摘要】：从尼罗罗非鱼脾脏组织中克隆获得新型免疫受体(NITR,Novel immune-type receptor)基因的编码区序列(Gen Bank登录号:KX989509;命名为On-NITR),该基因c DNA全长1 119 bp,ORF为1 026 bp,可编码341个氨基酸,理论分子量为37.38k D,等电点为8.28。通过NCBI BLAST比对发现罗非鱼NITR与其他已报道的物种NITR氨基酸序列相似度为27%-46%。氨基酸序列分析显示:On-NITR具有1个信号肽区域、2个胞外Ig-domain区、1个跨膜结构域,以及1个胞质尾区,该胞质尾区含有NITR典型的免疫受体酪氨酸抑制基序(ITIM)和一个ITIM类似基序itim,且具有较高的保守性。荧光定量PCR分析显示,On-NITR在健康尼罗罗非鱼组织中均有表达,在肠道、皮肤、肝脏表达水平较高,在胸腺、鳃、脾脏、心脏、脑组织中的表达量较低,在头肾组织中的表达量最低。
[Abstract]:The coding region of the novel immune receptor NITRN Novel immune-type receptor gene was cloned from spleen tissue of Tilapia niloticus. GenBank accession number: KX989509; On-NITRN; c DNA length 1 1119 BP; ORF 1 026 BP, encoding 341 amino acids. The theoretical molecular weight is 37.38 KD and the isoelectric point is 8.28. The similarity of NITR amino acid sequence between NITR and other reported species was 27-46 by NCBI BLAST comparison. Amino acid sequence analysis showed that there were one signal peptide region, two extracellular Ig-domain domains, one transmembrane domain and one cytoplasmic tail region. The cytoplasmic caudal region contains a typical NITR receptor tyrosine inhibitory motif and a ITIM similar motif itim. it is highly conserved. Fluorescence quantitative PCR analysis showed that the expression of On-NITR in the tissues of healthy Nile tilapia was higher in intestine, skin and liver, but lower in thymus, Gill, spleen, heart and brain. The lowest expression was found in the head and kidney tissues.
【作者单位】：广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室广东省教育厅水产经济动物病害控制重点实验室;
【基金】：广东省科技计划项目(2015A020209181) 2016年广东海洋大学优秀学位论文培育项目(201602)
【分类号】：S917.4

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