白羽王鸽L-FABP基因多态性与乳鸽生长和屠宰性能相关性研究

发布时间：2018-05-03 04:12

本文选题：白羽王鸽 + L-FABP　；参考：《安徽农业大学》2016年硕士论文

【摘要】：我国是肉鸽养殖大国,据统计,全国肉种鸽存栏量约4000万对,年产乳鸽约6亿只,年产值约100亿。目前我国肉鸽育种相对滞后,至今尚未有国家审定的肉鸽品种。加强肉鸽育种,是提升肉鸽业生产水平的关键。L-FABP基因参与脂肪酸的摄取、转运、线粒体氧化和酯化,在脂质代谢中起着重要的作用。本研究鉴定了该基因的遗传变异,并分析变异位点的遗传效应,为肉鸽分子育种提供参考。随机选择日龄相近的白羽王鸽28日龄乳鸽100只作为研究材料,依据NCBI(Gene ID:102086798)设计PCR引物7对,PCR产物覆盖调控区(-1162~0)及4个外显子和部分内含子区。通过将PCR扩增产物直接测序,在7对引物扩增产物中共发现了5个突变位点:100 GC位于外显子1上,G、C等位基因频率为0.38和0.62。1237TC位于外显子2上,T、C等位基因频率为0.67和0.33。1327 TC位于外显子2上,T、C等位基因频率为0.64和0.36,且100 GC、1237TC、1327 TC均为同义突变。SNP4(3838 CT)位于内含子3中,C、T等位基因频率为0.58和0.42。SNP5(3852 GT)位于内含子3上,G、T等位基因频率为0.58和0.42。变异位点遗传特性分析表明,各位点均呈现为中度多态(0.25PIC0.50),且均处于Hardy-Weinberg平衡内。对各位点与乳鸽生长性状和屠宰性能进行相关性分析表明:100 GC位点GG型(1.2%)与GC型(1.3%)腹脂率显著高于(P0.05)CC型(0.7%)。1327 TC位点TT型(17.7%)与TC型(16.6%)胸肌率显著低于(P0.05)CC型(19.3%),TT型(9.8%)与TC型(9.2%)腿比率显著低于(P0.05)CC型(10.8%)。其他位点与屠宰性状相关均不显著。综上所述,白羽王鸽L-FABP基因中共发现5个SNP,其中100 GC与腹脂率相关,1327 TC与胸肌率和腿比率相关。
[Abstract]:China is a big country in meat pigeon breeding. According to statistics, there are about 40 million pairs of pigeons stored in China, and about 600 million pigeons are produced annually, and the annual output value is about 10 billion. At present, the breeding of meat pigeon in China lags behind. Enhancing breeding of meat and pigeon is the key to improve the production level of meat pigeon. L-FABP gene is involved in fatty acid uptake, transport, mitochondrial oxidation and esterification, which plays an important role in lipid metabolism. In this study, the genetic variation of the gene was identified, and the genetic effect of the mutation site was analyzed, which provided a reference for molecular breeding of pigeon. A total of 100 young pigeons of 28 days old of similar age were randomly selected. According to NCBI(Gene ID: 1020 86798), 7 pairs of PCR primers were designed to cover the regulatory region of the NCBI(Gene and 4 exons and some intron regions. By sequencing the PCR products directly, A total of 5 mutation sites were found in 7 pairs of primer amplification products. The allele frequency was 0.38 in exon 1 and 0.38 in 0.62.1237TC on exon 2. The allele frequencies were 0.67 and 0.33.1327TC in exon 2. TGG C allele was located on exon 2 and TGG C allele was located on exon 2. The allele frequency was 0.67 and 0.33.1327TC was located on exon 2. Because the frequencies were 0.64 and 0.36, and the allele frequencies were 0.58 in intron 3 and 0.58 and 0.42 in intron 3, the alleles of 100 GCX 1237TCn1327TC were synonymous. The analysis of the genetic characteristics of the variation loci showed that all the sites showed moderate polymorphic 0.25PIC 0.50 and were in the Hardy-Weinberg equilibrium. The correlation analysis of growth traits and slaughtering performance of pigeons showed that the percentage of abdominal fat at GG (1.2) and GC (1.3) at the 1: 100 GC locus was significantly higher than that of P0.05CC type 0.77%. 1327 TC locus TT (17.7) and TC type 16.6) the pectoral muscle rate was significantly lower than that of P0.05CC (19.33TT = 9.8) and TC. The leg ratio was significantly lower than that of P0.05CC type 10.8. There was no significant correlation between other loci and slaughter traits. To sum up, five L-FABP genes were found in white feather pigeon, among which 100GC was correlated with abdominal fat rate and breast muscle rate and leg ratio.
【学位授予单位】：安徽农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S836

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