药用植物中鲨烯环氧酶基因的研究进展

发布时间：2018-05-03 19:40

本文选题：鲨烯环氧酶 + 基因克隆　；参考：《安徽医药》2016年03期

【摘要】：鲨烯环氧酶(SE)存在于内质网的微粒体中,在鲨烯转变为环氧化鲨烯的生物过程中作为生物催化剂起催化作用。而环氧化鲨烯是从鲨烯生成环阿屯醇、香树素、羊毛甾醇等的过程中重要的中间产物。SE的活性决定了环氧化鲨烯的生物合成的效率,继而,以环氧化鲨烯作为前体的各类甾体皂苷、三萜皂苷、甾醇等化合物的生物合成也随之受其影响,后续产物的产量取决于它的含量和活性。因此SE被认为是甾体皂苷生物合成途径中的一个非常重要的调控酶,已在多种植物中克隆出并进行了初步功能鉴定。该文概述几个重要药用植物的SE基因的克隆及原核表达,为从其他药用植物中克隆SE基因提供一定的参考依据及方法思路。
[Abstract]:Squalene cyclooxygenase (SEE) exists in the microsomes of endoplasmic reticulum and acts as a biocatalyst during the conversion of squalene to epoxide squalene. The activity of the intermediate product. SE determines the efficiency of the biosynthesis of the epoxidation squalene, which is the important intermediate product in the process of producing cycloarthol, vanillin, wool sterol and so on from the epoxidation squalene, and then the biosynthesis efficiency of the epoxidation squalene is determined. The biosynthesis of various steroidal saponins triterpene saponins sterols and other compounds with epoxidation squalene as precursor is also affected by the biosynthesis. The yield of subsequent products depends on its content and activity. Therefore, SE is considered to be a very important regulatory enzyme in the biosynthesis of steroidal saponins, which has been cloned and identified in a variety of plants. In this paper, the cloning and prokaryotic expression of SE gene of several important medicinal plants are summarized, which provides some reference basis and method for cloning SE gene from other medicinal plants.
【作者单位】：云南中医学院药学院;安徽中医药大学第一附属医院药学部;
【基金】：国家自然科学基金(No 81160502) 云南省自然科学基金(No 2011FZ153) 云南省教育厅自然科学基金(No2013J040)
【分类号】：Q943.2;S567

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