光温对叶菜型甘薯叶片花青素合成及相关酶基因表达的影响

发布时间：2018-05-03 20:21

本文选题：光照 + 温度　；参考：《核农学报》2017年01期

【摘要】：为了研究光照和温度对紫叶甘薯叶片花青素合成的影响及其调控机制,以福菜薯23号为材料,分别用不同的温度和光质处理后测定叶片花青素含量及相关酶基因的表达量。结果表明,叶片花青素含量及其生物合成结构基因不呈线性正相关;高温对福菜薯23号叶片花青素合成具有抑制作用,主要是下游基因DFR和F3H的表达受到抑制;与白光相比,蓝光促进福菜薯23号叶片花青素的合成,红光抑制花青素合成,混合光差异不大,尽管老叶的花青素含量高于新叶,但新叶花青素生物合成基因4CL、CHS、DFR和F3H表达量高于老叶片。本研究结果对提高叶菜型甘薯花青素含量具有指导意义。
[Abstract]:In order to study the effect of light and temperature on anthocyanin synthesis in purple sweet potato leaves and its regulation mechanism, the anthocyanin content and the expression of related enzyme genes in leaves were determined by different temperature and light quality treatments. The results showed that there was no linear positive correlation between anthocyanin content and biosynthesis structure genes in leaves, but high temperature inhibited anthocyanin synthesis in leaves of Fulai tuber 23, mainly the expression of downstream genes DFR and F3H, compared with white light. Blue light promoted anthocyanin synthesis in leaves of Fucai tuber 23, but red light inhibited anthocyanin synthesis, but the anthocyanin content in old leaves was higher than that in new leaves, but the expression of anthocyanin biosynthesis gene 4CLCHSfr and F3H in old leaves was higher than that in old leaves. The results of this study can be used to improve the anthocyanin content of leafy sweet potato.
【作者单位】：福建省农业科学院作物研究所/农业部南方薯类科学观测实验站;
【基金】：福建省省属公益类科研院所基本科研专项(2015R1026-1) 福建省农科院青年开放基金(2015QN-3);福建省农科院青年人才创新基金(2015CX-11)
【分类号】：S531

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