鸭MEF2A基因克隆及其mRNA在组织间的表达差异

发布时间：2018-05-04 07:18

本文选题：肌细胞增强因子A基因(MEFA) + 克隆　；参考：《农业生物技术学报》2017年01期

【摘要】：为了获取鸭肌细胞增强因子2A基因(myocyte-specific enhancer binding factor 2A gene,MEF2A)完整编码区(CDS)序列,同时对其在不同组织中的表达规律进行探究。本实验选取兴义鸭(Anas platyrhynchos)为研究对象,运用反转录PCR方法结合A/T克隆技术进行鸭MEF2A基因编码区克隆,运用q RT-PCR技术对其基于m RNA水平在组织间的表达差异进行研究。结果表明,实验成功获得了兴义鸭MEF2A基因完整编码区序列1 479 bp,并发现5个突变位点;q RT-PCR表明,MEF2A基因的表达虽在性别和组织间表达差异显著(P0.05或P0.01),但该基因主要在心肌和骨骼肌中表达,在平滑肌中的表达量最少。本实验结果为后期对鸭MEF2A基因原核表达载体构建及组织表达谱构建提供理论依据。
[Abstract]:In order to obtain the complete coding region of duck myocyte-specific enhancer binding factor 2A gene MEF2A, and to investigate its expression in different tissues. In this experiment, Anas platyrhynchos (Anas platyrhynchos) was selected as the research object, the reverse transcription PCR method combined with the A / T clone technique was used to clone the coding region of duck MEF2A gene, and Q RT-PCR technique was used to study the difference of expression between tissues based on m RNA level. The results show that The complete coding region sequence of MEF2A gene of Xingyi duck was obtained successfully. Five mutation sites, Q RT-PCR, indicated that the expression of MEF2A 2A gene was significantly different in sex and tissue (P0.05 or P0.01), but the gene was mainly expressed in myocardium and skeletal muscle. The expression in smooth muscle is the least. The results provided theoretical basis for construction of prokaryotic expression vector and tissue expression profile of duck MEF2A gene.
【作者单位】：贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室;贵州省动物遗传育种与繁殖重点实验室;贵州省毕节市农业产业办公室;
【基金】：贵州省科技合作计划联合基金项目(No.黔科合LH字(2015)7677号) 教育部科学技术研究重点项目(No.211168)
【分类号】：S834

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