固氮施氏假单胞菌bifA基因的功能分析

发布时间：2018-05-04 08:03

本文选题：施氏假单胞菌 + 环二鸟苷酸　；参考：《中国农业科技导报》2016年03期

【摘要】：环二鸟苷酸(c-di-GMP)是细菌中一种重要的第二信使,能够调控多种生理活动。C-di-GMP由两个GTP分子经环化酶(DGCs)合成,而被磷酸二酯酶(PDEs)降解,在铜绿假单胞菌中bifA基因为降解c-di-GMP的基因,参与了胞内c-di-GMP的浓度调节。以固氮施氏假单胞菌A1501中的bifA基因为研究对象,构建了bifA基因突变株;鉴定了bifA基因在c-di-GMP降解及生物膜形成中的功能。结果表明,bifA基因的突变造成了A1501菌中c-di-GMP的积累,同时增强了菌株的生物膜形成能力。此外bifA突变株运动能力大幅下降。由此推测bifA为c-di-GMP降解的关键基因,通过调节胞内c-di-GMP水平间接参与调控了生物膜形成以及菌体运动等生理活动。该结果为解析固氮微生物的信号传递及环境适应机制提供了理论基础。
[Abstract]:C-di-GMP is an important second messenger in bacteria, which can regulate many physiological activities. C-di-GMP is synthesized by two GTP molecules by cyclase DGCs, and degraded by phosphodiesterase (PDEs). The bifA group is a gene that degrades c-di-GMP in Pseudomonas aeruginosa. Participate in the concentration regulation of intracellular c-di-GMP. The bifA gene of Pseudomonas sp. A1501 was used to construct the mutant of bifA gene, and the function of bifA gene in c-di-GMP degradation and biofilm formation was identified. The results showed that the mutation of bifA gene resulted in the accumulation of c-di-GMP in A1501 strain and enhanced the ability of biofilm formation. In addition, the motor ability of bifA mutant decreased significantly. It is inferred that bifA is the key gene of c-di-GMP degradation, which indirectly participates in the physiological activities such as biofilm formation and cell movement by regulating the level of intracellular c-di-GMP. The results provide a theoretical basis for the analysis of signal transduction and environmental adaptation mechanism of nitrogen-fixing microorganisms.
【作者单位】：安徽农业大学生命科学学院;中国农业科学院生物技术研究所;
【基金】：国家自然科学基金项目(31470174,31570081) 广东省引进创新创业团队计划项目(2013S033) 安徽省高校自然科学研究重点项目(KJ2016A242)资助
【分类号】：Q933

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