沙柳SpsLAS基因克隆及生物信息学分析

发布时间：2018-05-04 08:26

本文选题：沙柳 + LAS　；参考：《分子植物育种》2017年02期

【摘要】：LAS基因对于植物侧生分生组织有重要影响。它被证实在拟南芥、番茄等草本科植物营养生长阶段参与腋生分生组织的形成,但在木本植物中的基因功能尚缺乏深入研究。本研究以沙柳为材料,利用RT-PCR技术成功克隆沙柳LAS基因,命名为SpsLAS,并对其进行生物信息学分析。结果表明,该基因CDS序列全长1 320 bp,编码439个氨基酸,预测蛋白分子量为49.876 8 k D,理论等电点(PI)为6.66,是亲水性蛋白;同源氨基酸序列比对结果表明沙柳LAS基因与番茄LS、拟南芥LAS等功能已确认的LS亚家族基因同源性较高,氨基酸相似性达50%以上;系统进化分析表明SpsLAS与杞柳和毛果杨亲缘关系更近;对LAS进行功能结构域分析得知LAS基因具有GRAS基因家族典型特征,属于GRAS基因家族LS亚家族;预测未发现跨膜结构和信号肽区域;SpsLAS蛋白二级结构包含173个α螺旋(Alpha helix),72个延伸链(Extended strand),194个无规则卷曲结构(Random coil);亚细胞定位预测该基因很可能定位于细胞核内。沙柳LAS基因的克隆对于研究木本植物萌蘖及其分枝机制有重要意义,同时丰富了木本植物中GRAS基因家族的相关研究。
[Abstract]:LAS gene plays an important role in the lateral meristem of plants. It has been proved that it is involved in the formation of axillary meristem in the vegetative growth stage of Arabidopsis, Tomato and other grass plants, but the gene function in woody plants has not been further studied. In this study, LAS gene was cloned by RT-PCR and named as SpsLAS. and its bioinformatics analysis was carried out. The results showed that the CDS sequence was 1 320 BP, encoding 439 amino acids. The predicted molecular weight of the predicted protein was 49.876 kD, and the theoretical isoelectric point (Pi) was 6.66. It was a hydrophilic protein. The results of homologous amino acid sequence alignment showed that there was a high homology of the LS subfamily genes of Salix LAS and tomato LSand Arabidopsis LAS and the amino acid similarity was more than 50%. Phylogenetic analysis showed that SpsLAS was more closely related to willow and poplar, and the functional domain analysis of LAS showed that LAS gene had typical characteristics of GRAS gene family and belonged to LS subfamily of GRAS gene family. The transmembrane structure and signal peptide region of SpsLAS protein were not found to contain 173 alpha helixs, 72 extended strands and 194 random coils, and the subcellular localization predicted that the gene might be located in the nucleus. The cloning of LAS gene from Salix wilfordii is of great significance for studying the mechanism of flowering and branching of woody plants and enriches the study of GRAS gene family in woody plants.
【作者单位】：内蒙古农业大学林学院;
【基金】：国家自然科学基金项目(31360187) 林木遗传育种国家重点实验室(中国林业科学研究院)开放课题基金(TGB2015001)共同资助
【分类号】：S793.9;Q943.2

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