绵羊Lpin2基因CDS区克隆与生物信息学分析

发布时间：2018-05-05 10:10

本文选题：绵羊 + Lpin基因　；参考：《中国畜牧杂志》2017年04期

【摘要】：本研究应用RT-PCR、5'-RACE、TA克隆技术获得绵羊Lpin2基因CDS区,并进行相关生物信息学分析,为其遗传特性及编码蛋白功能机制的研究提供基础数据。结果获得绵羊Lpin2基因2 754 bp,包括84 bp的5'UTR和2 670 bp的CDS区,编码889个氨基酸。生物信息学分析编码蛋白lipin2属不稳定的中性亲水脂溶性蛋白,存在跨膜域、2个O-糖基化位点和89个磷酸化位点,没有信号肽,定位于细胞质或细胞器中。存在由细胞质活性氧(ROS)主导的氧化还原机制形成的二硫键。二级结构包含高比例的环(80.54%)。蛋白N-末端和C-末端分别含有保守结构域Lipin_N和LSN2。绵羊与山羊、家牛、羊驼、猪、家犬、灵长类与啮齿类动物以及原鸡lipin2氨基酸序列同源,系统进化与其亲缘关系远近一致,Lpin2基因编码区进化保守。绵羊lipin2与lipin1氨基酸序列相似性相对较低(66%),可能与其有差异的功能活性有关。
[Abstract]:In this study, the CDS region of sheep Lpin2 gene was obtained by RT-PCR5 + -RACETA cloning technique, and the related bioinformatics analysis was carried out to provide basic data for the study of genetic characteristics and functional mechanism of encoding protein. Results the sheep Lpin2 gene was obtained, including 84 BP 5'UTR and 2 670 BP CDS region, encoding 889 amino acids. Bioinformatics analysis showed that the encoded protein lipin2 was an unstable neutral hydrophilic lipophilic protein with transmembrane domain, two O-glycosylation sites and 89 phosphorylation sites, with no signal peptide and located in the cytoplasm or organelle. There is a disulfide bond formed by redox mechanism dominated by cytoplasmic reactive oxygen species (Ros). The secondary structure contains a high ratio of 80. 54 rings. Protein N-terminal and C-terminal contain conserved domain Lipin_N and LSN2, respectively. Sheep and goat, domestic cattle, alpaca, pig, dog, primate and rodent, and proto-chicken lipin2 amino acid sequence are homologous, and the phylogenetic relationship of Lpin2 gene is conserved. The amino acid sequence similarity between lipin2 and lipin1 in sheep is relatively low, which may be related to its different functional activity.
【作者单位】：山西农业大学动物科技学院;天津农学院动物科学与动物医学学院;
【基金】：国家自然科学基金(31372292)
【分类号】：S826.2

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