RelA基因修饰的树突状细胞在大鼠角膜移植免疫排斥反应中的作用

发布时间：2018-05-05 12:41

本文选题：RelA基因 + 树突状细胞　；参考：《眼科新进展》2017年01期

【摘要】：目的探讨RelA基因修饰供体骨髓来源的树突状细胞(dendritic cells,DC)在大鼠角膜移植免疫排斥反应中的作用。方法以SD大鼠为供体,Wistar大鼠为受体,建立大鼠穿透性同种异体角膜移植动物模型。将60只受体大鼠随机分为4组,每组15只,分别为对照组(造模前7 d尾静脉注射PBS 1 mL)、mDC组(造模前7 d尾静脉注射mDC 1 mL,共5×10~6个细胞)、imDC组(造模前7 d尾静脉注射imDC 1 mL,共5×106个细胞)及RelA-DC组(造模前7 d尾静脉注射RelA修饰的DC 1 mL,共5×10~6个细胞)。术后每天观察角膜植片免疫排斥反应情况并记录角膜植片的存活时间;术后14 d每组随机处死5只受体大鼠取其角膜作组织病理学观察。结果 RelA-DC组角膜植片存活时间为(26.2±2.3)d,较对照组(11.4±1.3)d、mDC组(9.3±1.3)d及imDC组(19.4±2.6)d均明显延长,差异均有统计学意义(均为P0.05);imDC组的存活时间显著长于对照组、mDC组(均为P0.05);与mDC组比较,对照组植片的存活时间延长,差异有统计学意义(P0.05)。角膜植片组织病理学(HE染色)结果显示,对照组、mDC组角膜植片明显增厚、水肿,基质层胶原纤维排列紊乱,可见大量炎症细胞浸润以及新生血管;imDC组角膜植片呈不同程度水肿,基质层胶原纤维排列轻度紊乱,可见少量炎症细胞浸润;RelA-DC组角膜植片轻度水肿,基质层胶原纤维排列较规则,几乎没有单核细胞浸润。结论静脉输注RelA基因修饰的DC能抑制大鼠角膜移植术后免疫排斥反应的发生,明显延长角膜植片的存活时间。
[Abstract]:Objective to investigate the role of dendritic cells derived from RelA gene modified donor bone marrow in corneal allograft rejection in rats. Methods SD rats were used as donors and Wistar rats as recipients to establish penetrating corneal allograft models in rats. Sixty recipient rats were randomly divided into 4 groups with 15 rats in each group. The control group (PBS 1 mL / MDC group 7 days before modeling) and RelA-DC group (5 脳 10 6 cells imDC 1 mL group 7 days before modeling) and RelA-DC group (7 days before modeling) were divided into two groups: control group (7 days before modeling) and RelA-DC group (5 脳 10 6 cells) and 7 days before model making (imDC 1 mL, 5 脳 10 6 cells respectively) and RelA-DC group (7 days before the model was established), the control group (imDC 1 mL, 5 脳 10 6 cells) and the RelA-DC group (7 脳 10 6 cells before modeling) D caudal vein injection of RelA modified DC 1 mL, a total of 5 脳 10 ~ 6 cells. The immune rejection of corneal grafts was observed and the survival time of corneal grafts was recorded. On the 14th day after operation, 5 rats were randomly killed in each group for histopathological observation. Results the survival time of corneal grafts in RelA-DC group was 26.2 卤2.3 days, which was significantly longer than that in control group (11.4 卤1.3 days) and imDC group (19.4 卤2.6 days). The survival time of grafts in the control group was prolonged, and the difference was statistically significant (P 0.05). The results of HE staining showed that the corneal grafts in the control group were thickened, edematous, collagen fibers in stromal layer were disordered, a large number of inflammatory cells were infiltrated and corneal grafts in neovascularization group showed edema in varying degrees. The arrangement of collagen fibers in stromal layer was slightly disordered. A small amount of inflammatory cells infiltrated the corneal grafts in RelA-DC group. The collagen fibers in stroma layer were arranged regularly and almost no monocyte infiltration. Conclusion intravenous infusion of DC modified by RelA gene can inhibit the occurrence of immune rejection and prolong the survival time of corneal graft in rats.
【作者单位】：南方医科大学珠江医院眼科;
【基金】：国家自然科学基金面上项目(编号:81371067) 广东省自然科学基金(编号:S20130400137-13)~~
【分类号】：R779.65

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