基于高通量测序的玄参根部转录组学研究及萜类化合物合成相关基因的挖掘

发布时间：2018-05-05 21:10

本文选题：玄参 + 转录组　；参考：《中国中药杂志》2017年13期

【摘要】：该研究应用新一代测序技术Illumina HiSeq~(TM)4000在转录水平上对药用植物玄参根部进行测序,结合生物信息学方法开展基因功能注释和SSR位点搜索。通过测序,共获得65 602 036条原始序列。利用生物信息学软件拼接和组装序列,获得73 983条unigene,平均长度823 bp。序列同源性比较表明,56 389条unigene与其他物种具有不同程度的同源性。通过Swiss-Prot,GO,KEGG,COG比对注释,发现520条编码玄参次生代谢途径关键酶基因和191个相关转录因子。利用MISA软件在所有unigenes中共搜索到11 659个SSR位点,重复类型以二核苷酸为主。该研究所获得的参与次生代谢的关键基因可为研究玄参药用成分的生物合成和调控机制奠定基础,获得的大量SSR位点为后续研究玄参种质鉴定及遗传多样性研究提供参考。
[Abstract]:In this study, a new generation of sequencing technique, Illumina HiSeq~(TM)4000, was used to sequence the roots of medicinal plant, Illumina HiSeq~(TM)4000 at the transcriptional level, and to carry out gene functional annotation and SSR site search combined with bioinformatics. A total of 65,602,036 original sequences were obtained by sequencing. Using bioinformatics software to assemble and assemble the sequence, 73,983 Unigenees were obtained, with an average length of 823 BP. Sequence homology comparison showed that 56,389 unigene had different homology with other species. Based on the Swiss-ProtGG COG comparison, 520 key enzyme genes and 191 related transcription factors were found to encode the secondary metabolic pathway of Swiss-Protr. A total of 11 659 SSR loci were found in all unigenes by MISA software. The key genes involved in secondary metabolism obtained in this study can lay a foundation for the study of the biosynthesis and regulation mechanism of medicinal components of Radix basalariae, and a large number of SSR sites obtained can provide a reference for the further study on the germplasm identification and genetic diversity of Radix basalariae.
【作者单位】：重庆市中药研究院;中国中医科学院中药资源中心重庆分中心;重庆市中药良种选育与评价工程技术研究中心;
【基金】：重庆市“科技平台与基地建设”项目(cstc2014ptyjd10001) 中央本级重大增减支项目(2060302) 重庆市基本科研业务计划项目(2015cstc-jbky-01903) 重庆市基础与前沿研究计划项目(cstc2013jcyA10126)
【分类号】：S567.53

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