大肠杆菌侵染猪肠上皮细胞IPEC-J2后TAP1基因表达量的变化分析

发布时间：2018-05-06 06:49

本文选题：猪 + TAP基因　；参考：《畜牧与兽医》2017年08期

【摘要】：为了在细胞水平上探究抗原处理相关转运体1(TAP1)基因与仔猪大肠杆菌性腹泻间的关系以及在机体抗大肠杆菌侵染过程中是否发挥了作用,选用3种主要产肠毒素大肠杆菌(F18ab,F18ac和K88ac)刺激离体培养的猪肠上皮细胞(IPEC-J2),用实时荧光定量PCR检测3种大肠杆菌刺激后IPEC-J2细胞中TAP1基因表达量的变化。结果显示:IPEC-J2细胞经过3种大肠杆菌刺激后,TAP1基因在细胞中的表达量均显著高于对照组(P0.05),差异倍数分别是对照组的1.7,1.8和1.5倍;3种不同的大肠杆菌刺激,TAP1基因在IPEC-J2细胞中的表达量不存在显著差异(P0.05)。通过分析3种大肠杆菌侵染离体培养的猪肠上皮细胞后TAP1基因表达量的变化,在细胞水平上验证了TAP1基因的表达对仔猪抗细菌性腹泻具有一定的作用,为TAP1基因作为机体免疫调控的相关基因提供了一定的理论依据。
[Abstract]:In order to explore the relationship between antigen-processing associated transporter 1 (TAP1) gene and diarrhea caused by Escherichia coli in piglets at the cellular level, and whether it plays a role in the resistance to Escherichia coli infection, Three major enterotoxigenic Escherichia coli (E.coli) F18abP18ac and K88ac-stimulated in vitro porcine intestinal epithelial cells (IPEC-J2C) were used to detect the expression of TAP1 gene in IPEC-J2 cells stimulated by three kinds of Escherichia coli by real-time fluorescence quantitative PCR. The results showed that the expression of Tap1 gene in the cells stimulated by three kinds of Escherichia coli was significantly higher than that in the control group (P 0.05). The difference was 1.71.8and 1.5-fold of the control group, respectively. There was no significant difference in the expression of P0.05 in the cell. By analyzing the changes of TAP1 gene expression in cultured porcine intestinal epithelial cells infected with three kinds of Escherichia coli in vitro, it was proved that the expression of TAP1 gene had a certain effect on the resistance of piglets to bacterial diarrhea at the cellular level. It provides a theoretical basis for TAP1 gene as a related gene of immune regulation.
【作者单位】：扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室;江苏省种猪繁育和健康养殖工程技术研究中心;
【基金】：国家科技支撑计划(2015BAD03B01) 江苏省科技支撑计划重点项目(BE2014357) 苏州市科技攻关项目(SNG201628)
【分类号】：S858.28

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