桑树MaGte4基因的克

发布时间：2018-05-06 08:38

本文选题：桑树 + MaGte　；参考：《分子植物育种》2017年01期

【摘要】：Virp1基因是第一个被报道可以结合RNA的Bromodomain(BRD)蛋白,在马铃薯纺锤块茎类病毒(potato spindle tuber viroid,PSTVd)对宿主的感染及其复制中起重要作用。本研究根据NCBI登录的virp1基因序列和桑树基因组数据库设计引物,进行RT-PCR扩增和克隆测序,获得桑树的同源MaGte4基因长1 771 bp的序列。生物信息学分析表明,基因有3个外显子,CDS长1 683 bp,编码560个氨基酸,有与乙酰化组蛋白赖氨酸具有特异相互作用的BRD及其extra-terminal(BET)结构域,且该结构及其氨基酸组成在各物种间比较保守,桑树MaGte4具有和virp1蛋白非常相似的C-末端区域,RT-PCR分析基因在正常和花叶型萎缩病桑树叶片、叶柄和叶脉中均有表达,推测MaGte4的功能可能和Virp1相类似。
[Abstract]:Virp1 gene is the first reported protein that can bind to RNA. It plays an important role in host infection and replication of potato spindle tuber viroid virus. In this study, primers were designed based on the virp1 gene sequence registered by NCBI and the mulberry genome database. The homologous MaGte4 gene of mulberry was amplified by RT-PCR and cloned and sequenced. The homologous MaGte4 gene of mulberry was 1 771bp in length. Bioinformatics analysis showed that three exons of the gene had a length of 1 683 BP, encoding 560 amino acids, and the BRD and its extra-terminalBETs had a specific interaction with acetylated histone lysine. Moreover, the structure and amino acid composition of mulberry were conserved among species. RT-PCR analysis of the C-terminal region of mulberry MaGte4, which is very similar to virp1 protein, was expressed in the leaves, petioles and veins of mulberry with normal and mosaic atrophy. It is speculated that the function of MaGte4 may be similar to that of Virp1.
【作者单位】：安康学院陕西省蚕桑重点实验室;
【基金】：陕西省青年科技新星项目(No.2013KJXX-96) 陕西省教育厅重点实验室科研计划项目(No.13JS003)共同资助
【分类号】：S888.2;Q943.2

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