基因芯片技术筛选转SlNAC1基因拟南芥差异表达基因

发布时间：2018-05-06 14:06

  本文选题：差异表达 + 转基因　； 参考：《生物工程学报》2017年01期

【摘要】：研究已表明植物特有的一些NAC(NAM,ATAF1/2,CUC2)转录因子可提高植物抗逆性,利用基因芯片技术筛选转SlNAC1基因拟南芥与野生型拟南芥间差异表达基因,能够为研究转基因拟南芥非生物胁迫抗性相关基因提供依据。结果显示,在转SlNAC1基因拟南芥43 604个基因中有3 046个差异表达2倍以上的基因。对差异表达5倍以上基因经过GO富集度统计学分析表明,细胞组分相关基因占33.05%;分子功能相关基因占33.95%;生物学过程相关基因占33.00%。对差异表达2倍以上基因进行KEGG信号通路分析,结果表明有2 431个基因涉及到88个不同的信号通路。通过筛选获得转基因拟南芥非生物胁迫抗性相关候选基因,为后续研究NAC转录因子的下游基因及其调控网络的构建提供方向和理论支撑。
[Abstract]:Studies have shown that some plant specific transcription factors of NACMAMN ATAF1 / 2 CUC2 can improve plant stress resistance. Differential expression genes between transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana were screened by gene chip technique. It can provide the basis for the study of transgenic Arabidopsis Arabidopsis abiotic stress resistance related genes. The results showed that 3 046 of 43 604 genes were differentially expressed in Arabidopsis thaliana with SlNAC1 gene. Statistical analysis of differentially expressed genes by go enrichment showed that cell component related genes accounted for 33.05 genes, molecular function-related genes accounted for 33.95%, biological process genes accounted for 33.00%. The KEGG signaling pathway analysis of differentially expressed genes showed that 2 431 genes were involved in 88 different signal pathways. Candidate genes related to abiotic stress resistance of Arabidopsis thaliana were obtained by screening, which provided the direction and theoretical support for further research on downstream genes of NAC transcription factors and the construction of regulatory networks.
【作者单位】： 辽宁师范大学生命科学学院辽宁省植物生物技术重点实验室;
【基金】：国家自然科学基懶(No.31340052) 辽宁省教育厅科技研究项目(No.L201683655)资助~~
【分类号】：Q943.2
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本文编号：1852607