河北禽源致病性大肠杆菌耐药性、毒力基因及超广谱β-内酰胺酶分析

发布时间：2018-05-07 07:58

本文选题：禽 + 致病性大肠杆菌　；参考：《中国家禽》2017年12期

【摘要】：以83株鸡源大肠杆菌河北省分离株为研究对象,通过药敏试验和PCR方法测定其毒力基因及超广谱β-内酰胺酶(Extended-spectrumβ-lactamase,ESBLs)基因的分布。药敏试验结果显示,83株大肠杆菌对头孢菌素药物耐药严重,其中青霉素最高,为100%,头孢拉定为77.1%,头孢曲松钠为61.4%,头孢噻肟为60.2%;PCR结果显示,69株(83.1%)产β-内酰胺酶,其中blaCTX、blaOXA、blaTEM、blaCMY和blaSHV的阳性率依次为70%、25%、17%、6%和0,说明河北地区致病性大肠杆菌产ESBLs主要为blaCTX、blaOXA,且有28株同时携带两种以上基因;毒力基因检测结果显示,83株鸡源大肠杆菌毒力基因阳性率由高到低为iro N(54株,65.06%)、iutA(51株,61.45%)、iss(37株,44.58%)、hly T(28株,33.73%)和omp T(9株,10.84%),iro N、iutA的阳性率均大于60%,说明二者是河北禽源致病性大肠杆菌致病力的关键所在;有17株未检测到所测毒力基因的存在,但临床也表现为败血症,可能涉及其他毒力基因,需进一步探讨。
[Abstract]:The distribution of virulence gene and Extended-spectrum 尾 -lactamase (Extended-spectrum 尾 -lactamase) gene of 83 strains of Escherichia coli isolated from Hebei Province were determined by drug sensitivity test and PCR method. The results of drug sensitivity test showed that 83 strains of Escherichia coli were seriously resistant to cephalosporin, of which penicillin was the highest, cefradine was 77.1, ceftriaxone was 61.4 and cefotaxime was 60.22.The PCR results showed that 69 strains of ceftaxime produced 尾 -lactamases. The positive rates of CMY and blaSHV were 70% and 17.6%, respectively, indicating that the ESBLs production of pathogenic Escherichia coli in Hebei area was mainly blaCTX blaOXA, and 28 strains were carrying more than two genes at the same time. The results of virulence gene test showed that the positive rate of virulence gene of Escherichia coli was higher than 60, indicating that the positive rate of virulence gene was higher than that of iro Nu 54 strain (65.06) and omp T9 strain 10.84iro NiutA. The results showed that the positive rate of virulence gene was higher than 60%, indicating that the positive rate of virulence gene was higher than that of iro strain 65. 066.The results showed that the positive rate of virulence gene was higher than 600.The results showed that the positive rate of virulence gene of Escherichia coli was higher than that of omp T9 strain. The results showed that the positive rate of virulence gene was higher than 60%. The key to the pathogenicity of bacteria; 17 strains of virulence genes were not detected, but the clinical manifestations were septicemia, which may involve other virulence genes.
【作者单位】：河北农业大学动物医学院;唐山师范学院;河北大学生命科学学院;
【基金】：河北省科技计划项目(12226602)
【分类号】：S852.61

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