抗原致敏IL-27基因修饰的树突状细胞活化的CTL对食管癌细胞线粒体膜电位及凋亡的影响

发布时间：2018-05-07 11:58

本文选题：食管肿瘤 + 树突状细胞　；参考：《河北医科大学学报》2016年12期

【摘要】：目的研究IL-27基因转染的树突状细胞(dendritic cell,DC)活化CTL体内诱导食管癌细胞凋亡的影响。方法通过在裸鼠的移植瘤周注射食管癌细胞抗原致敏、IL-27基因修饰DC(DC_(IL-27+Ag))活化的特异CTL,采用流式细胞术检测细胞凋亡水平,荧光染料罗丹明123染色检测细胞线粒体膜电位水平并检测caspase-3蛋白表达水平。结果 5组之间凋亡率、膜电位水平、caspase-3表达差异均有统计学意义(P0.05):DC_(IL-27+Ag)、DCIL-27、DCnaive、Tnaive组凋亡率、caspase-3表达均高于PBS组,膜电位水平低于PBS组;DC_(IL-27+Ag)、DCIL-27、DCnaive组凋亡率、caspase-3表达均高于Tnaive组,膜电位水平低于Tnaive组;DC_(IL-27+Ag)、DC_(IL-27)组凋亡率、caspase-3表达均高于DCnaive组,膜电位水平低于DCnaive组;DC_(IL-27+Ag)组凋亡率、caspase-3表达均高于DCIL-27组,膜电位水平低于DCIL-27组。结论在荷瘤小鼠体内,经食管癌细胞抗原致敏、IL-27基因修饰的DC可活化特异性CTL产生较强的细胞毒作用,其机制可能是CTL通过降低食管癌细胞线粒体膜电位,启动内源性线粒体凋亡途径,激活caspase-3蛋白,从而诱导细胞凋亡。
[Abstract]:Objective to study the effect of IL-27 gene transfected dendritic cells (DC) on apoptosis of esophageal carcinoma cells induced by CTL. Methods specific CTLs were injected into the xenografts of nude mice, which were sensitized with IL-27 gene and modified with DC(DC_(IL-27. Flow cytometry was used to detect the level of apoptosis. The mitochondrial membrane potential and the expression of caspase-3 protein were detected by Rhodamine 123 staining. Results there were significant differences in the expression of caspase-3 and apoptosis between the five groups. The expression of caspase-3 was significantly higher in DCIL-27 / Ag-DCIL27 / Tnaive group than in PBS group, and the level of membrane potential was lower than that in PBS group. The expression of caspase-3 in DCIL-27 / DCIL-27DCnaive group was significantly higher than that in Tnaive group, and the expression of caspase-3 in DCIL-27 / DCnaive group was significantly higher than that in PBS group. The level of membrane potential was lower than that of Tnaive group. The expression of caspase-3 and caspase-3 in DCS group was higher than that in DCnaive group, and the expression of caspase-3 in DCnaive group was higher than that in DCIL-27 group, and the membrane potential level was lower than that in DCIL-27 group. Conclusion in tumor-bearing mice, IL-27 gene modified DC can activate specific CTL to produce strong cytotoxic effect, and the mechanism may be that CTL can reduce mitochondrial membrane potential of esophageal cancer cells. Activation of endogenous mitochondrial apoptosis pathway, activation of caspase-3 protein, thereby inducing apoptosis.
【作者单位】：河北省隆尧县医院胸外科;河北省涉县医院病理科;冀中能源峰峰集团有限公司总医院外科;冀中能源峰峰集团有限公司总医院皮肤性病科;河北医科大学第四医院胸外科;
【基金】：河北省医学科学研究重点课题(20110136)
【分类号】：R735.1

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