西藏小型猪IGF-1基因在不同生长发育阶段和不同组织器官中的差异表达

发布时间：2018-05-07 17:41

本文选题：IGF-基因 + 西藏小型猪　；参考：《中国实验动物学报》2016年06期

【摘要】：目的测定西藏小型猪的IGF-1基因在不同年龄阶段、不同组织中的表达分布情况,为未来的研究提供基础数据。方法采用实时荧光定量PCR技术,以GAPDH基因为内参照,检测了IGF-1基因在西藏小型猪不同组织和不同生长阶段间的表达变化。结果 (1)IGF-1基因表达的时序性研究表明在0、0.25、0.5、1、2岁时肌肉组织的表达量最低,在0、0.25、0.5岁时皮肤组织中表达量最高,在0.1、2、3岁时肝组织的表达量最高。(2)IGF-1基因在在7个不同组织中均有表达,0.25岁时其在表达丰度由高到低依次为:皮肤、肺、肝、肾、脾、心脏和肌肉。且在皮肤中的表达量最高且极显著高于其他各组织(P0.01)。同时在不同年龄阶段的表达中,IGF-1基因0.25岁时在各组织中的表达均达到峰值。结论西藏小型猪的IGF-1基因的表达呈现出明显的时空特异性。
[Abstract]:Objective to determine the expression and distribution of IGF-1 gene in different tissues of Tibetan miniature pigs at different ages and to provide basic data for future research. Methods the expression changes of IGF-1 gene in different tissues and growth stages of Tibet miniature pigs were detected by real-time fluorescence quantitative PCR and GAPDH as internal reference. Results the temporal study on the expression of IGF-1 gene showed that the expression of IGF-1 gene was the lowest in the muscle tissue at the age of 0. 25 and 0. 5 years old, and the highest in the skin tissue at 0. 25 and 0. 5 years old. The expression of IGF-1 gene was the highest in liver tissue at the age of 0.1 ~ 2 years. The expression of IGF-1 gene in 7 different tissues was found in the following order: skin, lung, liver, kidney, spleen, heart and muscle at the age of 0.25 years from high to low. The highest expression was found in the skin and was significantly higher than that in other tissues (P 0.01). At the same time, the expression of IGF-1 gene reached its peak in all tissues at 0.25 years of age. Conclusion the expression of IGF-1 gene in Tibetan miniature pigs has obvious temporal and spatial specificity.
【作者单位】：南方医科大学实验动物中心暨比较医学研究所;
【基金】：2015科研助手项目(C1032246) 广东省医学科研基金(编号:A2016255) 广东省科技计划项目(编号2016A030303008)
【分类号】：Q95-33;Q78

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