IL-17A、IL-17F基因多态性与接尘工人肺部炎症易感性的关系研究

发布时间：2018-05-09 13:03

本文选题：IL-A + IL-F　；参考：《四川大学学报(医学版)》2017年01期

【摘要】：目的探讨白细胞介素-17A(IL-17A)、白细胞介素-17F(IL-17F)基因多态性与接尘工人肺部炎症遗传易感性的相关性。方法选择67例肺部炎症患者作为病例组,126例相同粉尘暴露的健康工人作为对照组,现场监测其粉尘暴露情况;调查其人口学特征、职业信息等;采集外周静脉血,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析和验证IL-17A(G-197A)和IL-17F(7488T/C)两位点基因多态性,对IL-17A、IL-17F基因多态性与接尘工人肺部炎症易感性进行logistic回归分析。结果 1193例接尘工人中IL-17A(G-197A)基因型A/A、A/G、G/G分别有42例(21.76%)、95例(49.22%)、56例(29.02%);IL-17F(7488T/C)基因型T/T、T/C、C/C分别有128例(66.32%)、54例(28.98%)、11例(5.70%);各基因型的分布频率符合遗传学的HardyWeinberg平衡。2病例组IL-17A(G-197A)A/A基因型比例低于对照组(P0.05),而A/G、G/G基因型比例均高于对照组(P0.05);经年龄、吸烟情况、接尘浓度调整后,IL-17A(G-197A)A/G基因型(OR=5.03,P0.01)、G/G基因型(OR=3.35,P0.05)是接尘工人患肺部炎症的易感因素。3IL-17F(7488T/C)各基因型在病例组、对照组间分布差异无统计学意义。结论在相同的粉尘暴露条件下,与IL-17A(G-197A)A/A基因型相比,A/G、G/G基因型个体具有更高的患肺部炎症的风险,提示IL-17A(G-197A)A/G、G/G基因型的接尘个体可能对肺部炎症更具有易感性。
[Abstract]:Objective to investigate the relationship between the polymorphisms of interleukin-17 (IL-17A) gene and the genetic susceptibility to lung inflammation in dust-exposed workers. Methods Sixty-seven patients with pulmonary inflammation were selected as the control group, 126 healthy workers with the same dust exposure were selected as the control group, their dust exposure was monitored on the spot, their demographic characteristics and occupational information were investigated, and peripheral venous blood was collected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze and verify the polymorphism of IL-17AG-197A and IL-17Fr 7488T / C, and the logistic regression analysis was carried out between the polymorphism of IL-17Ahl-IL-17F gene and the susceptibility to lung inflammation in dust-exposed workers. Results among the 1193 workers exposed to dust, 42 of the 1193 workers were infected with IL-17An G-197A / G / G / G / G = 42. 766,95, respectively, with 56 cases with IL-17FN 7488Tc / C, with 128 cases of T / T / T / C / C = 66.3232 / 54 / 28.98A / G / G / G / G / G / G, respectively. The distribution frequency of each genotype was lower in the HardyWeinberg balanced 2 case group than in the control group. The distribution frequency of each genotype was lower in the HardyWeinberg balance 2 case group than in the contralateral genotype group (P < 0 05), and the distribution frequency of each genotype was consistent with that of the HardyWeinberg balance 2 case group. The IL-17A(G-197A)A/A ratio of the two genotypes was lower than that of the control group. The ratio of G / G genotype of A / G / G was higher than that of the control group (P < 0.05), and the age of the group was higher than that of the control group (P < 0.05). Smoking status, the genotype of IL-17An G-197A-1 / G was 5.03P0.01G / G genotype OR3.35 / P0.05), which was the susceptible factor of lung inflammation in dust-exposed workers. There was no significant difference in the distribution of different genotypes of IL-17An (7488T / C) between the control group and the case group. Conclusion compared with the IL-17A(G-197A)A/A genotype, the individuals with the same dust exposure had a higher risk of lung inflammation, suggesting that the individuals with IL-17AG-197A / G / G / G / G genotype might be more susceptible to pulmonary inflammation.
【作者单位】：四川大学华西公共卫生学院环境卫生与职业医学系;上海市浦东新区疾病预防控制中心;
【分类号】：R135.2

【参考文献】