大豆及豆制食品中转基因成分检测分析

发布时间：2018-05-09 14:19

本文选题：大豆制品 + 转基因成分检测　；参考：《食品科技》2017年09期

【摘要】：建立大豆及豆制食品中转基因成分分析的real-time PCR扩增体系和方法。选择市售的大豆、豆粉、豆干、豆腐、豆浆和腐竹等6种大豆及豆制食品,针对不同的样品探索高质量基因组DNA的提取方法,扩增大豆内参基因lectin进行质量检测。针对我国批准的3种主要转基因大豆品系(GTS40-3-2,A2704-12和MON89788)设计特异性的引物和探针,进行real-time PCR检测。购买3种转基因大豆品系的标准品作为质控对照。从该6种大豆及豆制食品中均提取到了高质量的基因组DNA,建立了稳定的针对外源基因特异性、结构特异性和品系特异性片段检测的real-time PCR扩增体系。成功建立了6种大豆及豆制食品中针对3种转基因大豆品系成分检测的real-time PCR方法。
[Abstract]:The real-time PCR amplification system and method for the analysis of transgenic components in soybean and soybean foods were established. Six kinds of soybean and soya bean foods, such as soybean, soybean powder, dried bean, bean curd, soybean milk and curd, were selected. The extraction methods of high quality genomic DNA were explored for different samples, and the lectin of soybean internal reference gene was amplified for quality detection. Specific primers and probes were designed for real-time PCR detection of three major transgenic soybean lines (GTS40-3-2A2704-12 and MON89788) approved in China. The standard samples of 3 transgenic soybean lines were purchased as quality control control. High quality genomic DNAs were extracted from the six soybean and soybean foods, and a stable real-time PCR amplification system was established for the detection of foreign gene specificity, structural specificity and strain specific fragments. A real-time PCR method for the detection of three transgenic soybean strains in 6 soybean and soybean foods was successfully established.
【作者单位】：广西壮族自治区疾病预防控制中心;
【基金】：广西壮族自治区疾病预防控制中心青年基金项目(GXCDC201505)
【分类号】：TS214.2

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