水稻GW5基因的1212-bp Indel变异对粒形的影响

发布时间：2018-05-10 07:49

本文选题：水稻 + 粒形　；参考：《江苏农业学报》2016年06期

【摘要】：为了探明粒宽基因GW5对水稻粒形的作用,本研究利用DNA序列分析技术和PCR技术,对已测序的稻属野生种、水稻微核心种质以及2007-2013年江苏省审定的粳稻品种中GW5的变异类型进行了鉴定。研究结果表明,野生稻都含有GW5基因的1212-bp序列,但DNA序列有着明显的分化。在水稻微核心种质的籼粳亚种中,相对于GW5插入型(Ins)而言,缺失型(Del)水稻品种有着较短的粒长、较宽的粒宽、较厚的粒厚、较小的长宽比和较重的千粒质量,并且GW5基因的变异对籼亚种的粒形和千粒质量的影响均达到极显著水平。江苏省近年审定的绝大部分粳稻品种中GW5基因为缺失型。GW5基因的1212-bp Indel变异对水稻粒形和千粒质量有着不同程度的影响,这些研究结果对利用GW5的等位基因进行水稻育种有着重要的指导价值。
[Abstract]:In order to find out the effect of grain width gene GW5 on the grain shape of rice, DNA sequence analysis and PCR technique were used to study the sequence of wild rice species. The variation types of GW5 in rice microcore collection and japonica rice varieties approved in Jiangsu Province from 2007 to 2013 were identified. The results showed that wild rice all contained the 1212-bp sequence of GW5 gene, but the DNA sequence had obvious differentiation. In the indica and japonica subspecies of rice microcore collection, compared with the GW5 insertion type, the missing rice varieties have shorter grain length, wider grain width, thicker grain thickness, smaller aspect ratio and heavier thousand grain mass. The variation of GW5 gene had significant effect on grain shape and thousand grain quality of indica subspecies. In most japonica rice varieties approved in Jiangsu Province in recent years, the 1212-bp Indel variation of the missing type. GW5 gene had different effects on the grain shape and 1000 grain mass of rice, because of the 1212-bp Indel variation of the missing type of GW5 gene. These results have important guiding value for rice breeding using GW5 alleles.
【作者单位】：扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心教育部植物功能基因组学重点实验室;
【分类号】：S511.035.3

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