兔神经介素U及其受体的基因克隆和组织表达的研究

发布时间：2018-05-10 09:43

本文选题：兔 + 神经介素U　；参考：《畜牧与兽医》2017年01期

【摘要】：神经介素U(NMU)是从猪脊髓分离得到的一种神经肽,主要为NMU-25和NMU-8。NMU通过与其2个G蛋白偶联受体(NMU-R1和NMU-R2)结合而发挥作用。本文用RT-PCR方法克隆了兔NMU及其受体基因片段,分析了其结构特点,研究其在兔体内的表达分布。克隆了兔NMU、NMU-R1和NMU-R2基因片段,其大小分别为443,366和203 bp,并将基因序列提交到Gen Bank数据库(Gen Bank ID:kp276160.1,km-236787.1和km-236788.1),表明克隆的兔NMU基因片段核苷酸序列与其他物种如大鼠、猪、牛和人相比有较高的同源性(78%~83%),NMU-R1基因核苷酸序列同源性为80%~87%,NMU-R2基因核苷酸序列同源性为82%~89%。兔NMU-R1和NMU-R2具有G蛋白偶联受体的跨膜结构。NMU、NMU-R1和NMU-R2基因分别定位于第2号、3号和1号染色体上。此外,NMU、NMU-R1和NMU-R2 mRNA在兔体内广泛表达。试验结果为深入研究NMU及其受体的生理功能奠定了基础。
[Abstract]:Interleukin-Umu (NMUU) is a neuropeptide isolated from porcine spinal cord, which is mainly composed of NMU-25 and NMU-8.NMU by binding to its two G protein-coupled receptors, NMU-R1 and NMU-R2. In this paper, NMU and its receptor gene fragments were cloned by RT-PCR method, their structural characteristics were analyzed, and their expression and distribution in rabbits were studied. Rabbit NMUNMU-R1 and NMU-R2 gene fragments were cloned, and their sizes were 443366 and 203bprespectively, and the gene sequences were submitted to Gen Bank database Gen Bank ID1: kp276160.1km-236787.1 and km-236788.1, indicating that the cloned rabbit NMU gene fragment nucleotide sequence was similar to that of other species such as rats and pigs. The nucleotide sequence homology of NMU-R1 gene between cattle and human is 80% and 87% respectively. The nucleotide sequence homology of NMU-R2 gene is 82%. Rabbit NMU-R1 and NMU-R2 have transmembrane structure of G-protein-coupled receptors. NMUNMU-R1 and NMU-R2 genes are located on chromosomes 2, 3 and 1, respectively. In addition, NMUNMU-R1 and NMU-R2 mRNA were widely expressed in rabbits. The results laid a foundation for further study on the physiological function of NMU and its receptors.
【作者单位】：南京农业大学动物医学院;
【分类号】：S829.1

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