基因修饰工具猪模型的建立及应用

发布时间：2018-05-11 12:09

本文选题：猪 + 基因修饰　；参考：《中国实验动物学报》2017年03期

【摘要】：基因修饰工具动物模型是指对已知基因加以修饰建立的,能够作为工具,从而帮助实现其他目的基因修饰动物模型。工具动物模型在生物学研究和生物医药开发中具有非常重要的作用,尤其是基因修饰动物模型在基因功能和信号通路等生物学基础研究方面做出了突出贡献。猪不仅是重要的农业经济动物,由于猪在器官结构、大小以及生理代谢方面与人更加接近,因此,猪也是比较理想的生物医药大动物模型。近几年,由于基因编辑技术的突破,工具猪模型的建立取得了引人注目的进展,人们已先后培育出了细胞多能性示踪工具猪模型,如细胞谱系示踪工具猪模型,可用于重组酶介导的基因交换工具猪模型,免疫缺陷猪模型以及可用于体内基因编辑的基因修饰工具猪模型,这些工具猪已逐渐得到推广应用。本文重点拟对具有广泛用途的基因修饰工具猪模型的研究进展和应用进行综述。
[Abstract]:The animal model of gene modification tool refers to the animal model which is established by modifying known genes and can be used as a tool to help achieve other purpose gene modification animal models. Tool animal models play a very important role in biological research and biomedical development, especially the genetically modified animal models have made outstanding contributions to basic biological research such as gene function and signal pathway. Pig is not only an important agricultural economic animal, but also an ideal biomedical animal model because it is closer to human in organ structure, size and physiological metabolism. In recent years, due to the breakthrough of gene editing technology, the establishment of tool pig model has made remarkable progress, people have developed a cell pluripotent tracer tool pig model, such as cell lineage tracing tool pig model. It can be used in recombinant enzyme mediated gene exchange tool pig model, immunodeficiency pig model and gene modification tool pig model which can be used for gene editing in vivo. These tools have been gradually popularized and applied. In this paper, the research progress and application of the widely used gene modification tool pig model are reviewed.
【作者单位】：中国科学院广州生物医药与健康研究院再生生物学重点实验室;
【基金】：国家自然科学基金(编号:81672317,81671121)
【分类号】：Q78;Q95-33

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