L-亮氨酸对猪流行性腹泻病毒感染仔猪血液生化指标和空肠免疫相关基因mRNA水平的影响

发布时间：2018-05-11 18:30

本文选题：亮氨酸 + 仔猪　；参考：《中国饲料》2017年12期

【摘要】：本试验旨在研究L-亮氨酸(Leu)对猪流行性腹泻病毒(PEDV)感染幼龄仔猪血液指标和空肠免疫相关基因m RNA表达量的影响,探索Leu对PEDV感染仔猪应激的缓解作用。选用30头体重相近的7日龄PEDV阴性仔猪,随机分为3个处理组:对照组、PEDV组和PEDV+Leu组,每个处理10个重复,每个重复一头猪。试验期10 d(7日龄~16日龄)。从第0天开始对PEDV+Leu组按2.8 g/kg体重饲喂Leu,而对照组和PEDV组均按4.1 g/kg体重饲喂L-丙氨酸。第7天早上对PEDV组和PEDV+Leu组采用口服的方式接种106.5TCID50的PEDV,同时对照组采用口服的方式接种等剂量的生理盐水,3 d后(16日龄)取血液和肠道样品。结果表明:与对照组相比,PEDV组仔猪血液中总胆红素(TBIL)水平、血小板分布宽度(PDW)及空肠黏膜CXCL11、REG3G m RNA表达量分别降低了20.92%、9.76%、56.00%、36.00%(P0.05),血液中白细胞(WBC)和淋巴细胞(LYM)数量、磷酸丝氨酸、酪氨酸和色氨酸浓度,及空肠粘膜GSTO2和HSPH1 m RNA水平分别提高了61.68%、27.66%、30.39%、50.54%、64.00%、75.00%、89.00%(P0.05);与PEDV组相比,PEDV+Leu组仔猪血液中组氨酸、天冬酰胺、尿素氮(BUN)浓度分别升高了55.43%、32.58%、83.33%(P0.05),而二胺氧化酶(DAO)含量、单核细胞(MONO)、单核细胞比(Monocyte Ratio,MONOR)、磷酸丝氨酸、谷氨酸、异亮氨酸、精氨酸、苏氨酸及空肠黏膜MX1和GSTO2 m RNA水平分别降低了28.80%、47.13%、43.87%、10.53%、42.12%、29.48%、29.82%、33.50%、32.11%、28.00%(P0.05)。因此,日粮中添加Leu提高了PEDV感染仔猪的免疫功能,缓解了PEDV感染仔猪的应激。
[Abstract]:The purpose of this study was to investigate the effects of L- leucine Leu on blood index and m RNA expression of jejunal immune-related gene in young piglets infected with porcine epidemic diarrhea virus (PEDVV), and to explore the role of Leu in alleviating stress in piglets infected with PEDV. Thirty 7-day-old PEDV negative piglets with similar body weight were randomly divided into three groups: control group and PEDV Leu group. The test period was 10 days, 7 days old and 16 days old. From the 0 th day, the PEDV Leu group was fed with 2.8 g/kg body weight, while the control group and the PEDV group were fed with L-alanine at 4.1 g/kg body weight. In the morning of the 7th day, PEDV group and PEDV Leu group were inoculated with 106.5TCID50 orally, while the control group was inoculated with the same dose of normal saline for 3 days and then took the blood and intestinal samples at the age of 16 days. The results showed that compared with the control group, the levels of total bilirubin TBIL, platelet distribution width (PDW), and the expression of CXCL11REG3G m RNA in jejunum mucosa decreased by 20.92and 9.760.The levels of WBCand LYM in blood, the amount of WBCand LYM in blood, and the expression of CXCL11REG3G m in the jejunum mucosa were decreased respectively compared with the control group. The concentrations of tyrosine and tryptophan, and the levels of GSTO2 and HSPH1 m RNA in jejunal mucosa increased by 61.68 and 27.666.660.390.50.54and 64.0075.00 respectively. Compared with PEDV group, the concentrations of histidine, asparagine and urea nitrogen bun in blood of piglets in PEDV Leu group increased by 55.433.33P0.05U, respectively, and the contents of diamine oxidase (DAO) increased respectively. Monocytes with Monocyte Ratiotropium monocytes, monocytes with monocytes, serine, glutamate, isoleucine, arginine, threonine and jejunum mucosal MX1 and GSTO2 m RNA decreased the levels of MX1 and GSTO2 m, respectively. Therefore, dietary Leu increased the immune function of PEDV infected piglets and alleviated the stress of PEDV infected piglets.
【作者单位】：动物营养与饲料安全湖北省协同创新中心动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院;
【基金】：国家自然科学基金(31572416) 武汉轻工大学科研项目(2016J08)
【分类号】：S858.28

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