7株鳗鲡致病性气单胞菌毒力基因胞外产物及其活性比较

发布时间：2018-05-13 11:14

本文选题：气单胞菌 + 胞外产物　；参考：《华中农业大学学报》2017年01期

【摘要】：为探讨致病性气单胞菌的致病力与其胞外产物和毒力因子之间的关系,联合利用PCR、SDS-PAGE、平板扩散及分子进化等方法对7株鳗源气单胞菌的胞外毒力因子进行比较研究。结果显示,该7株致病菌所含有的胞外毒力基因(hly、aer、ast、act、alt、ahpA、epr和exu)在种类和数量上差异显著,其中hly可能是鳗源气单胞菌重要的致病因子;不同致病菌的胞外蛋白在种类和数量上都存在差异,其中分子质量约为50.7ku的蛋白,其分泌量高且在鳗源气单胞致病菌中较保守;该7株致病菌胞外产物均具有溶血、酪蛋白酶和卵磷脂酶活性,胞外产物活性与毒力基因的分布一致;胞外毒素基因hly和ahpA具有较高的属间保守性,而exu则具有较好的种间特异性。
[Abstract]:In order to investigate the relationship between pathogenicity, extracellular products and virulence factors, the extracellular virulence factors of 7 strains of Aeromonas eel were studied by means of PCRX SDS-PAGE, plate diffusion and molecular evolution. The results showed that there were significant differences in the species and quantity of the extracellular virulence genes of the 7 strains, hly may be an important pathogenic factor of Aeromonas eel. The extracellular proteins of different pathogenic bacteria are different in species and quantity, in which the molecular weight is about the protein of 50.7ku, its secretion is high and it is conserved in the airborne bacteria of eel, and the extracellular products of the seven pathogenic bacteria all have hemolysis. The activities of caseinase and lecithin were consistent with the distribution of virulence genes, and the extracellular toxin genes (hly and ahpA) had high intergeneric conservation, while exu had better interspecific specificity.
【作者单位】：集美大学水产学院;鳗鲡现代产业技术教育部工程研究中心;集美大学水域环境与渔业资源监测中心;福建省海洋生物资源开发利用协同创新中心;
【基金】：国家自然科学基金项目(31172438和U1205123) 福建省科技厅重点项目(2013Y0089) 福建省教育厅项目(JA12195;JA15260) 厦门市科技局项目(3502Z20133015) 鳗鲡现代产业技术教育部工程研究中心开放基金项目(ZK2013004;RE201304;RE201306;RE201307) 校预研启动基金项目(ZQ2012007)
【分类号】：S943

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