灌喂氧化鱼油对黄颡鱼胃肠道黏膜胆固醇和胆汁酸合成途径基因差异表达的影响

发布时间：2018-05-13 13:12

  本文选题：氧化鱼油 + 黏膜　； 参考：《基因组学与应用生物学》2017年05期

【摘要】：分别提取灌喂氧化鱼油以及鱼油的黄颡鱼胃肠道肠道黏膜的总RNA。将氧化鱼油组、鱼油组总RNA等量混合后,采用RNA-Seq测序,用Trinity进行de novo拼接、组装,对单一基因进行功能注释;再将氧化鱼油组、鱼油组总RNA分别测序、注释后进行基因表达量分析,并计算氧化鱼油组对鱼油组单一基因的差异表达定量分析,以log_2(OFH/FH)值代表氧化鱼油组相对于鱼油组基因的差异表达量。基因差异表达显示,氧化鱼油导致黄颡鱼胃肠道黏膜组织中胆固醇、胆汁酸生物合成代谢途径的酶以及涉及胆固醇和胆汁酸吸收转运蛋白基因差异表达。黄颡鱼胃肠道黏膜中以乙酰辅酶A为原料的胆固醇生物合成途径关键酶基因差异表达显著下调,从细胞外吸收转运胆固醇的主要蛋白基因差异表达下调,显示灌喂氧化鱼油导致黄颡鱼胃肠道黏膜胆固醇合成能力下降、从其他组织吸收胆固醇的能力下降,血清胆固醇含量下降4.06%。灌喂氧化鱼油后,黄颡鱼胃肠道黏膜以胆固醇为原料的初级胆汁酸合成代谢的关键酶差异表达显著上调,而胆汁酸转运到细胞外和转运到肠腔的载体蛋白基因差异表达下调,肠道再吸收胆汁酸的转运载体蛋白基因差异表达下调及肝细胞从血液吸收转运的载体蛋白基因差异表达上调,而血清胆汁酸含量下降了20.00%。结果表明,胆汁酸的肠肝循环发生障碍,肠腔和血清胆汁酸含量下降、胆汁酸在细胞出现淤积的趋势。
[Abstract]:Total RNA was extracted from gastrointestinal mucosa of Pelteobagrus fulvidraco fed with oxidized fish oil and fish oil, respectively. After equal mixing of total RNA of oxidized fish oil group and fish oil group, RNA-Seq sequencing was used, de novo splicing with Trinity, assembly, function annotation of single gene, and total RNA sequence of oxidized fish oil group and fish oil group were sequenced respectively. After annotation, the gene expression was analyzed, and the differential expression of single gene in the oxidized fish oil group was calculated. The log2OFH / FH value was used to represent the differentially expressed amount of the gene in the oxidized fish oil group as compared with the fish oil group. Gene differential expression showed that oxidized fish oil resulted in differential expression of cholesterol, bile acid biosynthesis pathway, cholesterol and bile acid absorption transporter genes in gastrointestinal mucosa of Pelteobagrus fulvidraco. The differential expression of key enzyme genes in cholesterol biosynthesis pathway from acetyl coenzyme A in the gastrointestinal mucosa of Pelteobagrus fulvidraco was significantly down-regulated, and the differential expression of the main protein genes that absorb and transport cholesterol from the cells was down-regulated. The results showed that the ability of cholesterol synthesis in gastrointestinal mucosa of Pelteobagrus fulvidraco decreased, the ability to absorb cholesterol from other tissues decreased, and the content of serum cholesterol decreased 4.06%. After fed with oxidized fish oil, the differential expression of the key enzymes of primary bile acid synthesis and metabolism in the gastrointestinal mucosa of Pelteobagrus fulvidraco was significantly up-regulated, while the differentially expressed genes of the carrier protein which transported bile acid to the extracellular and intestinal lumen were down-regulated. The differential expression of transporter protein gene of intestinal reabsorbed bile acid was down-regulated and the expression of carrier protein gene of liver cells absorbed and transported from blood was up-regulated, while the content of serum bile acid decreased 20.00%. The results showed that the intestinal and hepatic circulation of bile acids was impaired, the contents of bile acids in intestinal cavity and serum were decreased, and the accumulation of bile acids was observed in the cells.
【作者单位】： 苏州大学基础医学与生物科学学院江苏省水产动物营养重点试验室;北京营养源研究所水产动物系统营养研究开放试验室;
【基金】：国家自然科学基金(K113409511)资助
【分类号】：S965.199
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本文编号：1883290