骨形态发生蛋白-7基因变异与太原地区汉族人群代谢综合征的关联研究

发布时间：2018-05-13 14:32

本文选题：代谢综合征 + 骨形态发生蛋白-7　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:探讨骨形态发生蛋白-7(BMP7)基因变异与太原地区汉族人群代谢综合征的关联性。方法:采用病例-对照研究方法,本研究选择2016.4-2017.1期间就诊于山西省人民医院门诊及住院部的代谢综合征患者90例,正常体检人群83例,均为太原地区汉族人群。在基因库NCBI中以NT 011362.11为参考序列选取具有代表性的BMP7中rs6025422、rs17480735基因位点,应用SNaPshot-PCR技术在所选太原汉族人群中进行基因型鉴定。结果:1.经Hardy-Weinberg平衡检验发现,位点rs6025422基因型AA、GG、AG的分布与位点rs17480745基因型AA、GG、GA的分布均符合Hardy-Weinberg平衡(P0.05),表示所选人群无选择性偏倚。2.代谢综合征组及对照组在性别、TC、UA方面差异无统计学意义(P0.05),两组在年龄、LDL-C、BMI、WHR方面差异有统计学意义(P0.05);两组在基因型rs6025422方面差异有统计学意义(P0.05),在基因型rs17480745方面差异无统计学意义(P0.05)。3.以代谢综合征为因变量,以年龄、BMI、位点rs6025422基因型、LDL-C、WHR为自变量进行多因素Logistic回归分析,年龄、LDL-C和基因型进入回归方程,年龄、LDL-C是代谢综合征的危险因素(年龄OR=1.090,95%CI:1.056-1.126,P=0.001;LDL-C OR=1.648,95%CI:1.094-2.484,P=0.049);而在基因型rs6025422中GG型是代谢综合征的保护因素(OR=-0.995,95%CI:0.179-0.763,P=0.018)。结论:1.BMP7基因位点rs6025422的变异与太原地区汉族人群代谢综合征有关联;2.年龄和LDL-C是太原汉族人群代谢综合征的危险因素,rs6025422变异位点的GG基因型是太原汉族人群代谢综合征的保护因素。
[Abstract]:Objective: to investigate the association between bone morphogenetic protein-7 (BMP7) gene variation and metabolic syndrome of Han nationality in Taiyuan. Methods: a case-control study was conducted. 90 patients with metabolic syndrome and 83 normal controls were selected from the outpatient and inpatient departments of Shanxi Provincial people's Hospital from June to July 2016.All of them were Han nationality in Taiyuan. The rs6025422 rs17480735 locus of the representative BMP7 was selected in the NCBI gene bank using NT 011362.11 as reference sequence, and the genotypes were identified by SNaPshot-PCR technique in the selected Taiyuan Han population. The result is 1: 1. By Hardy-Weinberg equilibrium test, it was found that the distribution of rs6025422 genotype AA-GGG AG and that of locus rs17480745 genotype AA-GGG GGG G G G of the rs6025422 genotype were in accordance with the Hardy-Weinberg equilibrium P 0.05, indicating that there was no selective bias. There was no significant difference in sex TCU UA between the metabolic syndrome group and the control group (P 0.05), but there was a significant difference between the two groups in age (P 0.05), genotype rs6025422 (P 0.05), and genotype rs17480745 (P 0.05 .3) in the two groups, and there was no significant difference between the two groups in terms of age and age (P < 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05). Taking metabolic syndrome as dependent variable, age BMIand locus rs6025422 genotype LDL-CHR as independent variable, multivariate Logistic regression analysis was carried out. Age, LDL-C and genotype entered the regression equation. Age LDL-C was the risk factor of metabolic syndrome (CI: 1.056-1.126P0. 001) LDL-C ORB was 1.64895 CI: 1.094-2.484P0.049, while in genotype rs6025422, GG type was the protective factor of metabolic syndrome, OR-0.99595CI0.179-0.763P0.018. Conclusion 1. The variation of rs6025422 in BMP7 gene is associated with metabolic syndrome of Han nationality in Taiyuan area. Age and LDL-C were the risk factors of metabolic syndrome in Taiyuan Han population. The GG genotype of rs6025422 mutation site was the protective factor of metabolic syndrome in Taiyuan Han population.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R589

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