水稻叶片早衰突变体ospls3的生理特征和基因定位

发布时间：2018-05-14 06:05

本文选题：水稻 + ospls　；参考：《作物学报》2016年05期

【摘要】：叶片衰老是作物叶片发育的最后阶段,功能叶早衰将影响作物产量和品质,因此,研究叶片早衰的分子与生理机制对于培育耐早衰优良品种具有重要意义。本研究利用~(60)Co-g辐射诱变籼稻N142,获得叶片早衰突变体ospls3,其叶片早衰始于分蘖期,最先表现为叶尖变褐及叶中上部出现褐色斑点,并向叶基部蔓延而使叶片枯死。生理分析表明,野生型剑叶的叶绿素含量显著低于倒二叶和倒三叶,而突变体的含量则分别低于野生型且依次显著降低;野生型剑叶、倒二叶和倒三叶间的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、O_2?~含量和H_2O_2含量基本不变,而突变体的这些活性和含量则依次显著升高;野生型剑叶、倒二叶和倒三叶的可溶性蛋白含量和过氧化氢酶(CAT)活性变化不显著,而突变体则依次降低。遗传分析表明,ospls3突变性状受1对隐性基因控制。借助图位克隆技术,将该基因定位于第12染色体长臂的RM6953与RM28753之间,物理距离为294 kb,该结果为进一步克隆Os PLS3基因并研究其功能奠定了基础。
[Abstract]:Leaf senescence is the last stage of crop leaf development, and functional leaf premature senescence will affect crop yield and quality. Therefore, it is important to study the molecular and physiological mechanism of leaf premature senescence in order to cultivate good varieties with early senescence resistance. In this study, the mutant ospls3 of leaf premature senescence was obtained by radiation mutagenesis of N142. The early senescence of leaves began at tillering stage, and the first appeared as browning of the tip and brown spots in the middle and upper part of the leaves, which spread to the base of the leaves and caused the leaves to wither and die. Physiological analysis showed that the chlorophyll content of wild flag leaf was significantly lower than that of the second leaf and the third leaf, while the content of mutant was lower than that of the wild leaf and decreased significantly in turn. The activity of superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA) content in the second leaf and the third leaf were basically unchanged, while the activity and content of these mutants increased significantly. The soluble protein content and catalase CAT activity of the second leaf and the third leaf did not change significantly, but the mutant decreased in turn. Genetic analysis showed that a pair of recessive genes controlled the mutant traits. With the help of map-based cloning technique, the gene was located between RM6953 and RM28753 on chromosome 12 with a physical distance of 294kb. The results laid a foundation for further cloning of Os PLS3 gene and study of its function.
【作者单位】：浙江大学农业与生物技术学院;浙江省农业科学院;
【基金】：国家自然科学基金项目(31271691) 国家转基因生物新品种培育重大专项(2013ZX08001-002)资助~~
【分类号】：S511

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