板栗过氧化氢酶基因CAT1的克隆及生物信息学分析

发布时间：2018-05-14 19:17

  本文选题：板栗 + CAT基因　； 参考：《食品工业科技》2017年13期

【摘要】：为研究板栗过氧化氢酶基因CAT1的功能,旨在为其采后贮藏中品质变化研究提供分子机理基础。以板栗为试材,提取总RNA,通过c DNA末端快速扩增技术(RACE),克隆到板栗CAT1基因c DNA全长,并对其进行生物信息学分析。结果表明,该基因序列全长1485 bp,包含一个1479 bp的开放阅读框,编码492个氨基酸。氨基酸序列分析表明,板栗CAT1与枣(Ziziphus jujuba)、烟草(Nicotiana tabacum)、陆地棉(Gossypium hirsutum)等植物过氧化氢酶CAT1氨基酸序列有较高的相似性。蛋白分析表明,该蛋白分子量为56947.31 Da,理论等电点6.65,属稳定性亲水蛋白;亚细胞定位于过氧化氢酶体,存在30个磷酸化位点,无信号肽。二级结构以α螺旋、无规则卷曲为主。保守结构域预测表明,该基因编码蛋白属于Catalase-like超家族。本研究克隆获得了板栗CAT1基因,为进一步研究该基因的生物学功能奠定了基础。
[Abstract]:In order to study the function of catalase gene CAT1 in Chinese chestnut, the purpose of this study was to provide a molecular basis for the study of quality change in postharvest storage. Total RNAs were extracted from Chinese chestnut and cloned into chestnut CAT1 gene c DNA by rapid amplification technique of c DNA terminal, and then analyzed by bioinformatics. The results showed that the gene was 1485 BP in length and contained a 1479 BP open reading frame encoding 492 amino acids. Amino acid sequence analysis showed that the amino acid sequence of catalase CAT1 in Chinese chestnut CAT1 and Ziziphus jujuba, Nicotiana tabacumum and Gossypium hirsutum were similar. Protein analysis showed that the protein had a molecular weight of 56947.31 Da and a theoretical isoelectric point of 6.65, which was a stable hydrophilic protein, and the subcell was located in catalase with 30 phosphorylation sites and no signal peptide. The secondary structure is 伪-helix and irregular crimp. The prediction of conserved domain showed that the gene encoded protein belonged to Catalase-like superfamily. In this study, the CAT1 gene of Chinese chestnut was cloned, which laid a foundation for further study of the biological function of the gene.
【作者单位】： 宁波大学食品科学与工程系应用海洋生物技术教育部重点实验室;
【分类号】：Q943.2;S664.2
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本文编号：1889136