武汉市输入性恶性疟原虫裂殖子表面蛋白1、2基因分型研究

发布时间：2018-05-15 00:37

  本文选题：恶性疟原虫 + 裂殖子表面蛋白　； 参考：《中国寄生虫学与寄生虫病杂志》2017年05期

【摘要】：目的了解武汉市输入性恶性疟原虫(Plasmodium falciparum)裂殖子表面蛋白1(merozoite surface protein 1,MSP1)和MSP2的等位基因型特征。方法于2010-2015年采集武汉市从非洲疟疾流行区回国人员中恶性疟现症患者血样,收集患者流行病学资料。提取患者血样中的恶性疟原虫DNA,采用巢式PCR分别扩增恶性疟原虫MSP1、MSP2基因型特异性片段,分析各等位基因型的构成比和不同感染来源地患者的基因型频数分布,分析重症患者不同基因型的相关性。不同基因型的重症患者比例差异采用χ~2检验。结果共采集输入性恶性疟患者血样175份,其中轻度症状患者血样137份,重症患者血样38份。巢式PCR结果显示,MSP1等位基因K1、RO33、MAD20型分别扩增出260~390、270和150 bp目的条带;MSP2等位基因3D7、FC27型分别扩增出200~330、330~500 bp目的条带。MSP1和MSP2基因均未检出的有9份。检出MSP1等位基因136份,检出率为77.7%,等位基因MAD20、K1、RO33、MAD20+K1、MAD20+RO33、K1+RO33、MAD20+K1+RO33型的构成比分别为5.1%(7/136)、37.5%(51/136)、20.6%(28/136)、5.9%(8/136)、4.4%(6/136)、16.9%(23/136)、9.5%(13/136)。检出MSP2等位基因143份,检出率为81.7%,FC27、3D7和FC27+3D7型的构成比为20.2%(29/143)、39.9%(57/143)、39.9%(57/143)。MAD20型和K1以南非最多,分别占10.5%(4/38)和34.2%(13/38);RO33型以东非最多,占2/7;FC27型东非和北非均未检出,南非占比最高,为18.4%(7/38);3D7型以东非最多,占4/7。MSP1基因MAD20、K1、RO33、MAD20+K1、MAD20+RO33、K1+RO33、MAD20+K1+RO33型的重症患者分别占1/7、19.6%(10/51)、32.1%(9/28)、1/8、4/6、13.0%(3/23)、46.2%(6/13),MAD20+RO33、MAD20+K1+RO33和RO33(P0.05),与其他4种基因型间差异有统计学意义(P0.05)。MSP2基因3D7、FC27、FC27+3D7型的重症患者比例分别为19.3%(11/57)、20.7%(6/29)、24.6%(14/57),各基因型间差异无统计学意义(P0.05)。结论武汉市输入性恶性疟原虫MSP1存在MAD20、Kl和R033等3种单基因型以及4种多基因型,以K1型和R033型为优势基因型;MSP2存在FC27、3D7和FC27+3D7型,3D7型的比例大于FC27型。
[Abstract]:Objective to investigate the allelic characteristics of merozoite surface protein (1(merozoite surface protein 1 MSP1) and MSP2 of Plasmodium falciparum in Wuhan. Methods Blood samples of patients with falciparum malaria from malaria endemic areas in Africa were collected from 2010 to 2015 in Wuhan. The plasmodium falciparum DNA was extracted from the blood samples of the patients, and the specific fragments of MSP1mSP2 genotypes were amplified by nested PCR. The composition ratio of each allele and the frequency distribution of the genotypes in patients with different infection sources were analyzed. To analyze the correlation of different genotypes in severe patients. The proportion of severe patients with different genotypes was tested by 蠂 2 test. Results 175 blood samples were collected from imported falciparum malaria patients, including 137 samples from patients with mild symptoms and 38 samples from patients with severe symptoms. The results of nested PCR showed that the MSP1 allele K1 / RO33 / MAD20 was amplified from 260h390270 and 150bp / mSP2 allele 3D7FFC27, respectively, and 9 of them were not detected in the target band. MSP1 and MSP2 genes were not detected. A total of 136 MSP1 alleles were detected, and the detection rate was 77.7.The ratio of MAD20K1 / MAD20K1and MAD20 RO33K1 / MAD20K1 RO33 was 5.1R / 7136C 37.5N = 51R / 7136C 20.6ng / 288136N 5.9 / 1364.4m = 16.6 / 166N = 16.923 / 136P / 169.136. There were 143 MSP2 alleles detected, and the detection rate was 81.7FC273D7 and FC27 3D7, and the ratio was 20.229 / 143D7 and 39.929 / 143Q / 39.90.57 / 143t0 / 39.9nb, the highest number were in South Africa (10.54r.38) and 34.21338P / RO33, respectively, in East Africa, and in 2 / 7FC27 East Africa and North Africa, the highest in South Africa, 18.47% in East Africa, 3D7% in East Africa, and 3D7% in East Africa. 鍗，

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