不同砧木对黄果柑果实有机酸含量和酸代谢相关酶活性及基因表达的影响

发布时间：2018-05-15 17:36

本文选题：黄果柑 + 砧木　；参考：《华北农学报》2016年04期

【摘要】：为了探究不同砧木对黄果柑果实有机酸含量、酸代谢相关酶活性及基因表达的影响,为黄果柑砧木的选择提供理论依据。以枳壳、红橘、香橙为砧木,黄果柑实生苗为对照进行研究。黄果柑果实以积累柠檬酸为主,香橙砧能够最有效地降低果实有机酸含量,成熟期比实生苗低24.35%;嫁接能够一定程度的降低CS活性,而对PEPC、MDH活性的影响较小;花后250~330 d,ACO和NADP-IDH活性均表现出香橙砧枳壳砧红橘砧CK的趋势;除实生苗外,黄果柑NADP-IDH表达水平与总酸含量间呈显著性负相关,而CS、MDH、ACO与总酸含量均未表现出显著相关性。香橙是黄果柑理想的砧木品种,砧木间果实有机酸差异是ACO和NADP-IDH活性共同作用的结果,且NADPIDH基因的表达可能是影响不同砧木黄果柑果实中有机酸含量的关键限制因子。
[Abstract]:In order to explore the effects of Different Rootstock on the organic acid content, the activity of acid metabolism related enzymes and the effect of gene expression, the theoretical basis for the selection of Citrus rootstock was provided. Orange fruit, orange and orange were used as the rootstock and citrus fruit as the control. Citrus fruit was mainly composed of citric acid, and the orange anvil could be reduced most effectively. The organic acid content of the fruit was 24.35% lower than that of the real seedling, and the grafting could reduce the activity of CS to a certain extent, but had little effect on the activity of PEPC and MDH, and the 250~330 D, ACO and NADP-IDH activity after the flower showed the trend of CK of orange anvil anvil anvil, and there was a significant negative phase between the NADP-IDH expression level of citrus and the total acid content except for the seedlings. CS, MDH and ACO have no significant correlation with the total acid content. Orange is the ideal rootstock of citrus, and the difference in organic acid between the rootstock is the result of ACO and NADP-IDH activity, and the expression of NADPIDH gene may be the key limiting factor of the organic acid content in the fruit of Different Rootstock.

【作者单位】：四川农业大学园艺学院;四川农业大学果蔬研究所;
【基金】：四川省科技支撑计划项目(2011NZ0034) 四川省科技厅基金项目(10ZC1454)
【分类号】：S666

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