小白杏脂氧合酶基因PaLOX的克隆与序列分析

发布时间：2018-05-15 22:35

本文选题：小白杏 + 脂氧合酶　；参考：《生物技术》2017年01期

【摘要】：[目的]克隆小白杏(Prunus armeniaca)PaLOX基因全长并对其进行序列分析。[方法]根据已有的ESTs序列,设计3'和5'端RACE引物,利用RT-PCR和RACE技术克隆小白杏PaLOX基因c DNA全长。[结果]PaLOX c DNA全长序列为2 723 bp,含有一个1 002 bp的ORF开放阅读框,编码334个氨基酸,含有PLAT高度保守的特异结构域,预测蛋白质的相对分子质量为26.558 1 k Da,推测理化等电点为8.77,分子式为C1689H2635N459O490S13;该蛋白主要表现为亲水性,α-螺旋和延伸链则散布于整个蛋白质中,空间结构不稳定,无信号肽和跨膜结构,可能存在于线粒体中。进化分析表明,PaLOX氨基酸序列与梅、碧桃、苹果、梨、草莓等蔷薇科聚为一类,相似性依次为98%、99%、78%、77%、63%。[结论]获得了编码小白杏脂氧合酶的基因PaLOX全长序列,登录号为KM067451。
[Abstract]:[objective] to clone Prunus armeniaca)PaLOX gene and sequence analysis. [methods] according to the existing ESTs sequence, the 3 'and 5' end RACE primers were designed and the full length of PaLOX gene c DNA was cloned by RT-PCR and RACE techniques. [results] the full-length PaLOX c DNA sequence was 2 723 BP, containing a 1 002 BP open reading frame of ORF encoding 334 amino acids and a highly conserved PLAT domain. The predicted molecular weight of the protein is 26.558 kDa, the predicted isoelectric point is 8.77, and the molecular formula is C1689H2635N459O490S13. The protein is mainly hydrophilic, the 伪 -helix and extension chain are scattered in the whole protein, and the spatial structure is unstable. No signal peptide and transmembrane structure may exist in mitochondria. Evolutionary analysis showed that the amino acid sequence of Palox was similar to that of the Rosaceae, such as Mei, Bitao, Apple, Pear, Strawberry, etc. [conclusion] A full-length PaLOX sequence encoding small white apricot lipoxygenase was obtained, and the accession number was KM067451.
【作者单位】：新疆农业科学院农产品贮藏加工研究所;新疆农业职业技术学院;阿克苏地区质量与计量检验所;
【基金】：国家科技支撑项目(No.2011BAD27B01-01-02) 新疆农科院青年基金项目(No.xjnkq-2014022)
【分类号】：S662.2

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