海岛棉GbWRKY40基因的克隆及特征分析

发布时间：2018-05-15 22:43

  本文选题：海岛棉 + GbWRKY　； 参考：《棉花学报》2017年04期

【摘要】：【目的】WRKY转录因子调控多种生物学进程,包括植物生长发育和应答多种环境胁迫。本研究旨在分析WRKY转录因子在海岛棉纤维发育中的功能。【方法】从海岛棉中克隆了1个WRKY转录因子基因GbWRKY40,进行同源性分析、多序列比对,利用荧光定量聚合酶链式反应分析其表达模式,通过构建酵母表达载体并转化酵母菌株AH109研究其转录激活活性。【结果】该基因c DNA全长1713 bp,5'非编码区长261 bp,3'非编码区长510 bp;开放阅读框长942 bp,编码313个氨基酸,预测相对分子质量约为34.138×103,等电点为8.46,包含5个外显子和4个内含子;其编码蛋白含有1个WRKY保守区(WRKYGQK)和1个锌指基序(C-X5-C-X23-H-X1-H),属于WRKY家族第Ⅱ类a组,包含3个核定位信号区,与陆地棉GhWRKY40同源性最高。GbWRKY40在根和开花后25 d纤维中表达量高,而且不具有转录激活活性。【结论】GbWRKY40可能参与调控棉纤维次生壁发育。
[Abstract]:Objective: WRKY transcription factors regulate many biological processes, including plant growth and response to multiple environmental stresses. The purpose of this study was to analyze the function of WRKY transcription factor in the fiber development of island cotton. [methods] A WRKY transcription factor gene GbWRKY40 was cloned from island cotton for homology analysis and multiple sequence alignment. Fluorescence quantitative polymerase chain reaction (FQPCR) was used to analyze its expression pattern. By constructing yeast expression vector and transforming yeast strain AH109, the transcriptional activation activity was studied. [results] the total length of c DNA was 1713 BP / 5'non-coding region 1713 BP / 3', and the length of open reading frame was 942 BP, encoding 313 amino acids. The predicted molecular weight is about 34.138 脳 103, the isoelectric point is 8.46, including 5 exons and 4 introns, and the encoded protein contains one WRKY conserved region WRKYGQK) and one zinc finger motif, C-X5-C-X23-H-X1-HN, which belongs to the second class a group of the WRKY family and contains three nuclear localization signal regions. GbWRKY40 had the highest homology with upland cotton GhWRKY40 and had high expression in root and 25 days after anthesis, and had no transcriptional activation activity. [conclusion] GbWRKY40 may be involved in the regulation of cotton fiber secondary wall development.
【作者单位】： 新疆农业大学农学院/新疆农业大学农业生物技术重点实验室;
【基金】：基金项目:新疆维吾尔自治区青年科技创新人才培养工程优秀青年科技人才(qn2015yx012)
【分类号】：S562
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本文编号：1894298