斑马鱼胚胎发育过程中miR-9、miR-219及Dicer基因的时序性表达

发布时间：2018-05-16 00:44

本文选题：胚胎 + 发育　；参考：《解剖学报》2017年04期

【摘要】：目的探讨斑马鱼胚胎发育过程中miR-9、miR-219及Dicer基因时序性表达规律。方法取正常发育至受精后4、8、12、16、20、24、36、48和72 h(hpf)斑马鱼胚胎各约40枚,用Trizol法分别提取总RNA,茎环法反转录为c DNA,用Real-time PCR检测胚胎miR-9、miR-219及Dicer表达量。结果 miR-9、miR-219及Dicer表达水平在胚胎发育过程中呈现峰谷变化,不同发育阶段之间总的差异有统计学意义(P0.05)。与胚胎发育全程的基因平均表达水平相比,miR-9在8 hpf之前表达量较低(P0.05),36 hpf后表达量持续增高,48 hpf和72 hpf时表达量显著高于平均水平(P0.01)。miR-219在8 hpf之前表达量也处于较低水平(P0.05),20 hpf后迅速增高,24hpf时达到峰值,并持续到48 hpf(P0.01)。Dicer在胚胎早期(4 hpf)就有明显表达(P0.05),随后表达水平迅速下降,16 hpf时达到低点(P0.05),然后逐渐回升,直至胚胎末期。结论斑马鱼胚胎发育过程miR-9、miR-219及Dicer时序性表达有一定规律,miR-9主要在胚胎后期表达,miR-219主要在胚胎中期表达,Dicer主要在胚胎早期表达,其次在胚胎后期,提示miR-9、miR-219及Dicer参与胚胎发育调控过程。
[Abstract]:Objective to investigate the temporal expression of miR-9 miR-219 and Dicer genes in zebrafish during embryonic development. Methods about 40 zebrafish embryos from normal development to fertilization were collected. Total RNAs were extracted by Trizol method. The results were reversed into c DNAs by stem ring method. The expression of miR-9 miR-219 and Dicer in embryos were detected by Real-time PCR. Results the expression of miR-9 miR-219 and Dicer showed peak and valley changes during embryonic development, and the total difference between different developmental stages was statistically significant (P 0.05). Compared with the average level of gene expression during embryonic development, the expression level of mmiR-9 before 8 hpf was lower than that at 48 hpf and 72 hpf after P0.05 + 36 hpf, which was significantly higher than that of P0.01MR-219 before 8 hpf. After 20 hpf, the peak value of P0. 05 and P0. 05 was reached at 24hpf. The expression of P0.05 was observed at 48 hpf(P0.01).Dicer and reached a low point at 16 hpf, and then increased gradually until the end of embryo. Conclusion the expression of miR-9 miR-219 and Dicer in zebrafish during embryonic development has a certain regularity. The expression of miR-219 is mainly in the middle stage of embryo, followed by the later stage of embryo. These results suggest that miR-9 miR-219 and Dicer are involved in the regulation of embryonic development.
【作者单位】：温州医科大学公共卫生与管理学院预防医学系;
【基金】：国家自然科学基金(30972509) 温州市科技计划(Y20140218)
【分类号】：Q344

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