邢台地区致鸡卵黄性腹膜炎大肠杆菌血清型鉴定、毒力基因检测及耐药性分析

发布时间：2018-05-16 12:29

本文选题：大肠杆菌 + 卵黄性腹膜炎　；参考：《中国预防兽医学报》2017年03期

【摘要】：为分析邢台地区致鸡卵黄性腹膜炎大肠杆菌(E.coli)分离株的血清型、毒力基因和耐药性,本实验采用常规鉴定方法和16S rRNA PCR的方法,从邢台地区不同养鸡场46份患卵黄性腹膜炎的40周龄~45周龄蛋鸡病料组织中分离鉴定出37株E.coli。人工感染1日龄的雏鸡,结果显示32株分离菌为致病性E.coli,采用玻板凝集法检测其血清型分布,结果显示定型的27株致病性E.coli分离株共属于11个血清型,以O_(78)、O_(148)、O_2及O_8为优势血清型。采用PCR方法检测32株致病性E.coli分离株14种毒力基因,其中irp2、fyuA、ompT、Iss~a、iucD~a、iutA、iroN、hlyF、fimC毒力基因检出率最高,分别为100%、96.9%、93.8%、86.7%、84.3%、71.9%、65.7%、56.3%和50%,其余毒力基因检出率较低。耐药性分析结果显示,32株致病性E.coli分离株仅对头孢克肟、左氧氟沙星、阿奇霉素、头孢曲松和氟苯尼考5种药物高度敏感,对其他药物存在不同程度的耐药性。本研究为防治致病性E.coli引起蛋鸡卵黄性腹膜炎提供了实验依据。
[Abstract]:In order to analyze the serotype, virulence gene and drug resistance of E. coli isolated from chicken egg yolk peritonitis in Xingtai area, routine identification method and 16s rRNA PCR method were used in this study. 37 strains of E. coli were isolated and identified from the tissues of 40 weeks and 45 weeks old layers infected with egg yolk peritonitis in different chicken farms in Xingtai area. The results showed that 32 strains of virulent E. coli were infected artificially. The distribution of serotype was detected by glassy plate agglutination method. The results showed that the 27 strains of pathogenicity E.coli were classified into 11 serotypes, and the predominant serotypes were O _ (78) O _ (78) O _ (1) O _ (2) and O _ (+) _ (8). PCR method was used to detect 14 virulence genes of 32 pathogenic E.coli isolates, among which Ip2fyuAompTnus iucus iuhlyfimC had the highest virulence gene detection rate, which were 93.89.84.34.34.34.37.The detection rates of other virulence genes were 56.3% and 50.3%, respectively, and the other virulence genes were lower than those of the control group (P < 0.05). The virulence genes of the two isolates were 96.84.34.34.37% and 55.3%, respectively, and the positive rates of the other virulence genes were lower than those of the control group (P < 0.05). The results of drug resistance analysis showed that 32 strains of pathogenic E.coli were highly sensitive to cefixime, levofloxacin, azithromycin, ceftriaxone and florfenicol, and had different drug resistance to other drugs. This study provides experimental evidence for the prevention and treatment of egg yolk peritonitis caused by pathogenic E.coli.
【作者单位】：河北科技师范学院河北省预防兽医学重点实验室;
【基金】：河北省现代农业产业技术体系蛋鸡产业创新团队岗位(HBCT2013090203) 河北省研究生创新资助项目(2016~2017) 河北省科技支撑项目(12220412D) 河北省高等学校科学技术研究项目(2015064)
【分类号】：S852.61

【相似文献】