丹参SmbHLH37基因的克

发布时间：2018-05-16 12:48

本文选题：丹参 + 碱性螺旋-环-螺旋(bHLH)　；参考：《农业生物技术学报》2017年06期

【摘要】：碱性螺旋-环-螺旋(basic helix-loop-helix,bHLH)转录因子家族在植物的生长发育、响应非生物胁迫等方面发挥着重要的调控作用。为了进一步丰富丹参(Salvia miltiorrhiza)中该家族成员的研究,寻找新的参与调控丹参次生代谢产物合成相关的bHLH成员,本研究基于丹参转录组和基因组数据库,分别从cDNA和gDNA水平克隆获得SmbHLH37基因序列。该基因序列长1 506 bp,包含1个27 bp的内含子序列,1 479 bp完整的CDS(GenBank登录号:KP257470.1),编码492个氨基酸。Blast P分析结果显示,SmbHLH37与芝麻(Sesamum indicum)等植物的bHLH3同源性较高;SmbHLH37蛋白与拟南芥(Arabidopsis thaliana)bHLH转录因子家族的分子进化树结果显示,SmbHLH37与拟南芥bHLH转录因子At JAM3(A.thaliana jasmonate-associated MYC2-like3)亲缘关系最近。亚细胞定位结果显示,SmbHLH37蛋白主要定位在细胞核中。qRT-PCR分析结果显示,SmbHLH37在叶中的表达量最高,花中最低;用脱落酸(abscisic acid,ABA)和机械诱导处理后,该基因的表达短时间呈上调趋势;同时,SmbHLH37在Sm MYC2过表达株系OE-8和OE-12中的表达量显著上调(P0.05)。研究结果为进一步开展SmbHLH37功能研究提供了基础资料。
[Abstract]:Basic helix-loop-helixhlhh (basic helix-loop-helixhh) transcription factor family plays an important role in plant growth and development, response to abiotic stress, and so on. In order to further enrich the family members of Salvia miltiorrhizae and find new bHLH members involved in regulating the synthesis of secondary metabolites of Salvia miltiorrhizae, this study was based on the transcriptome and genomic database of Salvia miltiorrhizae. The sequence of SmbHLH37 gene was obtained from cDNA and gDNA. The gene sequence is 1 506bpand contains a 27bp intron sequence of 1 479bp complete CDS(GenBank accession number: KP257470.1, encoding 492 amino acids .Blast P analysis results show that SmbHLH37 has high bHLH3 homology with Arabidopsis thaliana and other plants such as Sesamum indicum. The molecular phylogenetic tree of Arabidopsis thaliana)bHLH transcription factor family showed that SmbHLH37 was closely related to Arabidopsis bHLH transcription factor at JAM3(A.thaliana jasmonate-associated MYC2-like 3. The results of subcellular localization showed that the SmbHLH37 protein was mainly located in the nucleus. QRT-PCR analysis showed that the expression of SmbHLH37 was the highest in the leaves and the lowest in the flower, and the expression of SmbHLH37 was up-regulated in a short time after abscisic acidmatine (ABA) and mechanical induction. At the same time, the expression of SmbHLH37 in Sm MYC2 overexpression lines OE-8 and OE-12 was significantly up-regulated (P0.05). The results provide basic data for further research on SmbHLH37 function.
【作者单位】：陕西师范大学西北濒危药材资源开发国家工程实验室/药用植物资源与天然药物化学教育部重点实验室;
【基金】：国家自然科学基金项目(No.31670299)
【分类号】：Q943.2;S567.53

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