马氏珠母贝载脂蛋白基因Pm-APOL3克

发布时间：2018-05-17 14:29

本文选题：马氏珠母贝 + 载脂蛋白L　；参考：《基因组学与应用生物学》2016年07期

【摘要】：Apolipoprotein L3(APOL3)是新发现的一种高密度脂蛋白,属于载脂蛋白L基因家族的成员之一,具有运载脂类物质进行生化代谢的功能。本研究利用c DNA末端快速扩增技术获得了马氏珠母贝APOL3基因(Pm-APOL3)c DNA全长序列,分析其序列及功能特征,检测Pm-APOL3基因在不同组织的表达,并分析了金黄壳色群体与养殖群体类胡萝卜素含量与Pm-APOL3基因表达量的相关性。结果表示,Pm-APOL3基因c DNA全长874 bp,开放阅读框(ORF)为357 bp,编码118个氨基酸,包含475 bp的5'UTR,42 bp的3'UTR,相对分子量为12.795 5 ku,等电点为4.71。该蛋白质的不稳定指数为34.09,属于稳定蛋白质,疏水性分析表明其亲水性平均系数为0.74,属疏水蛋白,信号肽预测结果表示其不含信号肽,为非分泌蛋白。荧光定量PCR分析表明Pm-APOL3基因在鳃、外套膜、闭壳肌与肝胰脏均有表达,其中肝胰脏表达量显著大于其它三个组织(p0.05)。金黄壳色群体类胡萝卜素含量与Pm-APOL3基因表达量均显著大于养殖群体(p0.05)。本研究结果表明Pm-APOL3是参与马氏珠母贝类胡萝卜素代谢重要基因。
[Abstract]:Apolipoprotein L3 / APOL3 is a newly discovered high density lipoprotein, which belongs to the apolipoprotein L gene family and has the function of carrying lipid substances for biochemical metabolism. In this study, the full-length of APOL3 gene Pm-APOL3C DNA was obtained by rapid amplification of c DNA terminal, and its sequence and functional characteristics were analyzed, and the expression of Pm-APOL3 gene in different tissues was detected. The correlation between carotenoid content and Pm-APOL3 gene expression was analyzed. The results showed that the total length of c DNA of Pm-APOL3 gene was 874bp.The open reading frame (ORF) was 357bp, encoding 118 amino acids, containing 475bp of 5- UTR4bp, with a relative molecular weight of 12.795 5kuand an isoelectric point of 4.71. The instability index of this protein is 34.09, which belongs to stable protein. The hydrophobic average coefficient of the protein is 0.74, which is hydrophobic protein. The prediction result of signal peptide indicates that the protein is non-secretory protein without signal peptide. Fluorescence quantitative PCR analysis showed that Pm-APOL3 gene was expressed in Gill, mantle, obturator muscle and hepatopancreas. The expression of Pm-APOL3 gene in hepatopancreas was significantly higher than that in the other three tissues. The content of carotenoid and the expression of Pm-APOL3 gene in golden shell color population were significantly higher than that in cultured population. The results suggest that Pm-APOL3 is an important gene involved in carotenoid metabolism of Eucalyptus martensii.
【作者单位】：广东海洋大学水产学院;广东省珍珠养殖与加工工程技术研究中心;
【基金】：广东省科技厅项目“近江牡蛎三倍体优质苗种及马氏珠母贝新品种培育技术研究与开发”(2013B020201002) 广东省海洋与渔业局科技专项“马氏珠母贝优质抗逆选系苗种规模化繁育与示范养殖”(Z2014009) 2015年广东海洋大学“海之帆-起航计划”大学生科技创新项目“马氏珠母贝类胡萝卜素与相关基因表达量在关联分析”;2015年广东海洋大学水产学院创新项目“马氏珠母贝类胡萝卜富集机理分析”共同资助
【分类号】：S917.4

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