过表达P57KIP2基因对滋养层细胞生物学行为的影响

发布时间：2018-05-17 15:56

本文选题：子痫前期 + PKIP基因　；参考：《实用妇产科杂志》2017年08期

【摘要】：目的:探究P57KIP2基因过表达之后对滋养层细胞生物学行为的影响。方法:采用实时荧光定量(q PCR)及Western Blot方法检测人绒毛膜滋养层细胞(HTR8-/SVneo)过表达P57KIP2基因后mRNA和蛋白水平的表达变化;用Transwell方法检测HTR8-/SVneo细胞过表达P57KIP2基因后迁移、侵袭能力生物学行为的变化;CCK8检测HTR8-/SVneo细胞过表达该基因后增殖能力的变化。采用免疫荧光检测HTR8-/SVneo细胞过表达该基因后,抗凋亡蛋白Bcl2的表达变化。结果:滋养层细胞过表达P57KIP2基因后,其mRNA及蛋白水平均明显增高(P0.05);P57KIP2基因过表达后滋养层细胞的迁移、侵袭数量均明显增高,差异有统计学意义(P0.05);滋养层细胞的细胞相对吸光值48小时后明显增高(P0.001);过表达P57KIP2基因后滋养层细胞中抗凋亡蛋白Bcl2表达增高(P0.05)。结论:P57KIP2基因可能会增加滋养层细胞迁移、侵袭以及增殖能力,P57KIP2基因可能参与调控滋养细胞的凋亡过程。
[Abstract]:Objective: to investigate the effect of overexpression of P57KIP2 gene on the biological behavior of trophoblast cells. Methods: the expression of mRNA and protein in human chorionic trophoblastic cells (HTR8-SVneoa) was detected by real-time fluorescence quantitative PCR and Western Blot methods, and the expression of mRNA and protein in HTR8-/SVneo cells after overexpression of P57KIP2 gene was detected by Transwell method. The change of biological behavior of invasion ability and the change of proliferative ability of HTR8-/SVneo cells after overexpression of the gene were detected by CCK8. The expression of anti-apoptotic protein Bcl2 was detected by immunofluorescence after overexpression of the gene in HTR8-/SVneo cells. Results: after overexpression of P57KIP2 gene in trophoblast cells, the levels of mRNA and protein in trophoblast cells were significantly increased, and the migration and invasion of trophoblast cells were significantly increased after overexpression of P0.05 and P57KIP2 gene. The difference was statistically significant (P 0.05), the relative absorptivity of trophoblast cells was significantly increased after 48 hours, and the expression of anti-apoptotic protein (Bcl2) in trophoblast cells was increased after overexpression of P57KIP2 gene. Conclusion the P57KIP2 gene may increase the migration, invasion and proliferation of trophoblast cells. P57KIP2 gene may be involved in the regulation of trophoblast apoptosis.
【作者单位】：四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室;
【基金】：国家自然科学基金项目(编号:81471463) 四川省科技厅课题(编号:2014JY0158)
【分类号】：R714.244

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