控制水稻叶形基因CLF1的图位克隆

发布时间：2018-05-18 16:07

本文选题：水稻 + 突变体clf　；参考：《生物技术通报》2017年09期

【摘要】：分蘖、株高、植株形态是水稻理想株型的3个主要农艺性状。目前对水稻理想株型的分子调控机制认识还非常有限。而叶片形态建成是决定植株形态特征的主要因子,鉴定控制水稻叶片形态建成的关键基因至关重要。本研究通过筛选水稻突变体库,获得一份光合效率显著提高的突变体(curly leaf 1,clf1),其形态学特征表现为叶片适度卷曲,经石蜡细胞学切片发现,突变体clf1近轴面的泡状细胞明显增多是导致叶片卷曲和光合效率提高的主要原因。利用图位克隆,将CLF1基因缩小在2个分子标记In Del51与In Del57之间,该区间包含44个基因。通过生物信息学分析,确定了LOC_OS02G45250为CLF1的候选基因。对LOC_OS02G45250基因进行测序,在clf1突变体中,LOC_OS02G45250基因的第六外显子缺失20 bp,造成编码产物提前终止。该基因与已报道的水稻卷叶基因Roc5(Rice outermost cell-specific gene5)为等位基因,Roc5编码产物为一个具有GL2类同源异型结构域的转录因子,不同于已报道的突变体oul1(对应于Roc5基因),突变体clf1农艺性状表现优良,具有较大的生产应用潜力。
[Abstract]:Tillering, plant height and plant morphology are the three main agronomic characters of rice ideal plant type. At present, the understanding of molecular regulation mechanism of ideal plant type in rice is very limited. Leaf morphogenesis is the main determinant of plant morphological characteristics, and identification of key genes controlling leaf morphogenesis in rice is very important. In this study, a mutant curly leaf _ 1 clf _ 1 was obtained by screening the rice mutants bank. The morphological features of the mutant were moderate curl of leaves, which were found by paraffin cytological sections. The obvious increase of vesicular cells in the paraxial surface of the mutant clf1 was the main reason for the increase of leaf curl and photosynthetic efficiency. The CLF1 gene was reduced to two molecular markers between in Del51 and in Del57 by map cloning. The region contained 44 genes. LOC_OS02G45250 was identified as a candidate gene for CLF1 by bioinformatics analysis. The LOC_OS02G45250 gene was sequenced and the exon 6 of the OS02G45250 gene was missing 20 BP in the clf1 mutant, which resulted in the early termination of the encoding product. This gene is a transcription factor with a homologous domain of GL2, which is the allele Roc5 encoding product from the reported rice leaf coiling gene Roc5(Rice outermost cell-specific gene5. Different from the reported mutants oul1 (corresponding to Roc5 gene), the mutant clf1 has good agronomic characters and has great potential for production and application.
【作者单位】：中国农业科学院生物技术研究所;
【基金】：国家自然科学基金项目(31471166) 中国-巴西作物生物技术与种质资源联合研究项目(2014DFA31550)
【分类号】：Q943.2;S511

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