新疆南疆驴源H3N8亚型EIVHA基因序列分析

发布时间：2018-05-19 06:49

  本文选题：新疆南疆 + 驴　； 参考：《西北农业学报》2017年03期

【摘要】：为明确新疆南疆驴是否感染马流感病毒(Equine influenza virus,EIV)及驴源EIV HA基因特征,根据GenBank登录的EIV基因序列,设计合成1对引物,采集新疆南疆地区2个驴屠宰点33份驴肺组织样品,采用RT-PCR扩增驴源EIV HA基因片段,并对扩增产物进行测序与序列分析。结果表明,中国新疆南疆驴源EIV A/donkey/Xinjiang/1/2015(H3N8)株HA基因长1 704bp,编码567个氨基酸。同源性分析结果显示,与国内外H3N8亚型EIV参考毒株核苷酸和氨基酸同源性分别为88.9%~99.9%和88.3%~99.8%,与国内毒株和哈萨克斯坦毒株在一个进化分支内。与GenBank数据库中唯一1个驴源毒株A/donkey/Xinjiang/5/2007(H3N8)的比对结果显示,2个毒株裂解位点、糖基化位点、抗原位点和受体结合位点氨基酸完全一致,只是错位2个氨基酸。确定新疆南疆存在驴感染EIV,可能为国内疫情的延续或周边国家传入。
[Abstract]:In order to find out whether the donkeys are infected with equine influenza virus and the EIV HA gene of donkeys in southern Xinjiang, a pair of primers were designed and synthesized according to the EIV gene sequence registered by GenBank, and 33 donkey lung tissue samples were collected from two donkey slaughtering sites in southern Xinjiang. Donkey EIV HA gene fragment was amplified by RT-PCR and sequenced. The results showed that the HA gene of the donkeys' EIV A / R / Xinjiang / 1 / 2015 H3N8 strain was 1704 BP and encoded 567 amino acids. The results of homology analysis showed that the nucleotide and amino acid homology of the reference strains of H3N8 subtype EIV were 88.999% and 88.3% respectively, which were in an evolutionary branch with domestic strains and Kazakstan strains. The results of comparison with the only donkey virus strain A / R / Xinjiangr / 5 / 2007 H3N8 in GenBank database showed that the amino acids of the two strains were identical in cleavage site, glycosylation site, antigen site and receptor binding site, but only 2 amino acids were translocated. It is confirmed that the existence of donkey infection in southern Xinjiang may be a continuation of the domestic epidemic situation or the introduction of neighboring countries.
【作者单位】： 塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室;
【基金】：兵团塔里木畜牧科技重点实验室开放课题(HS2014011)~~
【分类号】：S852.65
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本文编号：1909121