新疆维吾尔族癫痫患者中拉莫三嗪所致药疹与HLA基因的相关性研究

发布时间：2018-05-19 06:55

  本文选题：癫痫 + 拉莫三嗪　； 参考：《安徽医科大学》2016年硕士论文

【摘要】：目的近年来在国内外人群中的研究表明,HLA-A*3001,HLA-A*3101,HLA-B*1502,HLA-B*38,HLA-B*5801等位基因可能与拉莫三嗪所致药疹存在相关性,而在新疆维吾尔族人群中尚没有该类研究的报道,因此本研究旨在探讨HLA-A*3001,HLA-A*3101,HLA-B*1502,HLA-B*38,HLA-B*5801等位基因与新疆维吾尔族癫痫患者中拉莫三嗪(LTG)所致药疹的关联性。方法在新疆地区28例使用LTG的维吾尔族癫痫患者(其中10例出现斑丘疹(M PE),18例未出现皮肤不良反应),以及30例维吾尔族健康受试者中,采用序列特异性引物-聚合酶链式反应技术(PCR-SSP),进行HLA-A*3001,HLA-A*3101,HLA-B*1502,HLA-B*38,HLA-B*5801等位基因筛选,并采用SPSS 19.0统计学软件进行统计学分析,通过卡方检验比较各组间HLA基因频率的差异性,通过t检验比较各组间的计量资料,P0.05代表组间具有显著性差异,并计算比值比(Od ds Ratio,OR)及其95%置信区间(Confidence Interval,CI),作为HLA基因与拉莫三嗪所致皮肤不良反应相关性的评价指标。结果在LTG-MPE和LTG耐受组之间,HLA-A*3001(P=0.936,OR=2,95%CI 0.236-16.928),HLA-A*3101(P=0.585,OR=4.25,95%CI 0.334-54.066),HLA-B*1502(P=0.875,OR=0.818,95%CI 0.066-10.117),HLA-B*38(P=0.875,OR=0.818,95%CI 0.066-10.117),HLA-B*5801(P=0.585,OR=4.25,95%CI 0.334-54.066)等位基因阳性率不存在显著性差异;在LTG-MPE和健康受试者之间,HLA-A*3001(P=0.543,OR=3.5,95%CI 0.424-28.915),HLA-A*3101(P=0.783,OR=2.25,95%CI 0.318-15.901),HLA-B*1502(P=0.811,OR=1.364,95%CI 0.112-16.577),HLAB*38(P=0.893,OR=0.491,95%CI 0.051-4.698),HLA-B*5801(P=0.298,OR=7.25,95%CI 0.581-90.545)等位基因阳性率也不存在显著性差异。结论在新疆维吾尔族癫痫患者中,HLA-A*3001,HLA-A*3101,HLA-B*1502,H LA-B*38,HLA-B*5801等位基因与LTG-MPE不存在显著相关性,但是由于样本量较少,统计学意义有限,因此仍需大样本研究进一步证实。
[Abstract]:Objective in recent years, studies in domestic and foreign population indicated that the allele of HLA-AHA-3101T / HLA-B3101Hla / B ~ (3102N) / HLA-B ~ (5801) allele may be associated with drug rash induced by lamotrigine, but there is no report of this kind of allele in Xinjiang Uygur nationality population (Uygur), and there is no report of this kind of allele in Xinjiang Uygur population. The aim of this study was to investigate the association between HLA-A3101T alleles and drug rash induced by lamotriazine (LTG) in Xinjiang Uygur patients with epilepsy. Methods among 28 Uygur epilepsy patients who were treated with LTG in Xinjiang area (10 cases with MPEP, 18 cases with no adverse skin reaction) and 30 healthy Uygur subjects were included in this study. Sequence specific primer polymerase chain reaction (PCR-SSPN) technique was used to screen the HLA-Bn5801 alleles of HLA-AHA-3101T, and the SPSS 19.0 statistical software was used to analyze the frequency of HLA gene. The frequency of HLA gene in each group was compared by chi-square test. T test was used to compare the significant difference between the two groups (P0.05), and to calculate the ratio of Ods Ratioor) and 95% confidence interval (CI), which was used as an index to evaluate the correlation between HLA gene and adverse skin reaction induced by lamotrigine. 缁撴灉鍦↙TG-MPE鍜孡TG鑰愬彈缁勪箣闂，

本文编号：1909141