油菜株高QTL定位、整合和候选基因鉴定

发布时间：2018-05-20 00:19

本文选题：甘蓝型油菜 + 株高　；参考：《中国农业科学》2017年17期

【摘要】：【目的】通过对油菜株高进行多环境QTL定位并与已报道的油菜株高QTL和植物株高基因分别进行整合和比对分析,揭示油菜株高的遗传结构和候选基因并为其分子改良提供依据。【方法】以油菜优良品种中双11(测序)和No.73290(重测序)衍生的含184个单株的Bna ZNF2群体为试验材料。首先,对Bna ZNF2群体进行基因型分析,利用Joinmap 4.0软件构建了一张含803个分子标记的高密度遗传图谱。其次,对F2:3和F2:4家系进行连续两年(2010—2011)两点(武汉和西宁)田间试验和表型鉴定。然后,利用Bna ZNF2群体的基因型数据和F2:3以及F2:4家系的株高表型数据,采用Win QTLCart 2.5软件的复合区间作图法进行QTL检测。最后,利用元分析的方法采用Bio Mercator软件对不同环境中检测到的株高QTL进行整合。【结果】对两年两点环境下分别检测到的株高QTL进行整合总共得到5个株高QTL的位点:q PH.A2-1、q PH.A2-2、q PH.C2-1、q PH.C3-1和q PH.C3-2,分布于A2、C2和C3染色体上,解释2.6%—55.6%的表型方差。其中,q PH.A2-1和q PH.A2-2只在武汉检测到,而q PH.C2-1、q PH.C3-1和q PH.C3-2只在西宁检测到。位于C2连锁群的主效QTL-q PH.C2-1只在西宁被重复检测到,而且LOD值、加性效应和贡献率(分别为23.4、-16.0和55.6%)均高于前人报道,是目前发现的效应最大的一个油菜株高QTL。基于油菜基因组物理图谱对本研究和已报道的油菜株高QTL和植物株高基因分别进行整合和比对分析,获得了一个由183个QTL和287个候选基因组成的相对完整的油菜株高遗传结构图。其中,有18个株高QTL簇能在不同研究中被共同检测到,分布在A1、A2、A3、A6、A7、A9、C6和C7染色体上。另外,本研究定位到的5个油菜株高QTL的物理位置和已报道的油菜株高QTL均不重叠,因而是新的株高QTL位点。其中,q PH.A2-2、q PH.C3-1和q PH.C3-2物理区间内总共找到了15个株高同源基因,而11个在2个亲本中存在序列变异,被选作候选基因进行进一步研究。【结论】QTL定位和整合获得5个油菜株高QTL,均为首次报道而且都只在武汉或西宁被检测到。其中位于C2连锁群的主效QTL效应值超过以往报道,表现出极强的QTL与环境的互作。通过与已报道的油菜株高QTL和植物株高基因分别进行整合和比对分析,较为全面地揭示了油菜株高的遗传结构和候选基因,生物信息学分析还鉴定到11个位于本研究定位到的3个株高QTL区间内的候选基因。
[Abstract]:[objective] to analyze rapeseed plant height by multi-environment QTL mapping and integration with reported QTL and plant height genes in rapeseed (Brassica napus L.). The genetic structure and candidate genes of rapeseed plant height were revealed and the basis for its molecular improvement was provided. [methods] the Bna ZNF2 population containing 184 individual plants derived from superior rapeseed varieties Zhongshuang 11 (sequencing) and No.73290 (re-sequencing) was used as experimental material. Firstly, the genotypes of Bna ZNF2 population were analyzed and a high-density genetic map containing 803 molecular markers was constructed by using Joinmap 4.0 software. Secondly, the field trials and phenotypic identification of F2: 3 and F2: 4 families were carried out for two consecutive years (Wuhan and Xining). Then, the genotypic data of Bna ZNF2 population and the phenotypic data of plant height of F2: 3 and F2: 4 families were used to detect QTL using the compound interval mapping method of Win QTLCart 2.5 software. Finally, Using meta-analysis method, Bio Mercator software was used to integrate the QTL of plant height detected in different environments. [results] five QTL loci of plant height QTL were obtained by integrating the QTL of plant height detected in two years and two years respectively. A total of 5 loci of QTL with height of plant were obtained. PH.A2-1 / Q PH.A2-2 / Q PH.C2-1Q PH.C3-1 and Q PH.C3-2, distributed on chromosomes A _ 2C _ 2 and C _ 3, Explain 2.6- 55.6% of phenotypic variance. Q PH.A2-1 and Q PH.A2-2 were detected only in Wuhan, while Q PH.C2-1 PH.C3-1 and Q PH.C3-2 were detected only in Xining. The main effect QTL-q PH.C2-1 located in C2 linkage group was only repeatedly detected in Xining, and the LOD value, additive effect and contribution rate (23.4mg-16.0 and 55.6) were higher than those reported by previous reports. So far, it was the largest effect found in rapeseed plant height QTL. Based on the physical map of rapeseed genome, QTL and plant height genes of rape were integrated and compared, respectively. A complete genetic map of rapeseed plant height consisting of 183 QTL and 287 candidate genes was obtained. Among them, 18 plant height QTL clusters could be detected in different studies, and were distributed on the chromosomes A1, A2A2A2A3, A6, A7, A9, C6 and C7. In addition, there was no overlap between the physical location of QTL and the reported QTL of rape plant height, so it was a new QTL locus of rapeseed height. A total of 15 high homologous genes were found in the physical range of Q PH.A2-2Q PH.C3-1 and Q PH.C3-2, while 11 of them had sequence variations in 2 parents. [conclusion] five rapeseed plant height QTLs were obtained by QTL mapping and integration, all of which were reported for the first time and were only detected in Wuhan or Xining. The dominant QTL effect in C2 linkage group is higher than that in previous reports, showing a strong interaction between QTL and environment. The genetic structure and candidate genes of rape plant height were revealed by integrating and comparing with the reported genes of rape plant height QTL and plant height, respectively. Bioinformatics analysis also identified 11 candidate genes located in the QTL region of 3 plants located in this study.
【作者单位】：中国农业科学院油料作物研究所;浠水县农业局油料作物推广站;
【基金】：基金项目:国家油菜产业技术体系(CARS-13) 中国农业科学院科技创新工程(CAAS-ASTIP-2013-OCRI) 国家公益性科研院所基本科研业务费(1610172017001) 湖北农业科技创新中心
【分类号】：S565.4

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