同源盒基因A13下调对小细胞肺癌细胞增殖、周期、凋亡和耐药性的影响

发布时间：2018-05-21 02:29

本文选题：小细胞肺癌 + 同源盒基因A　；参考：《中国病原生物学杂志》2017年12期

【摘要】：目的探讨小细胞肺癌细胞同源盒基因A13(HOXA13基因)的表达及其下调前后对小细胞肺癌细胞株H146和H446增殖、细胞周期、凋亡以及耐药性的影响。方法利用实时荧光定量PCR(RT-qPCR)法和Western blot法检测小细胞肺癌细胞株H69、H69AR、H1688、H146、H446及人支气管上皮细胞16HBE中HOXA13mRNA和蛋白的表达水平;应用RNAi技术下调HOXA13基因高表达细胞株H146和H446中HOXA13基因表达水平,采用CCK8法和流式细胞术检测HOXA13基因细胞增殖、周期、凋亡及耐药性变化。结果与16HBE细胞相比H69、H1688、H146、H446四种细胞中HOXA13基因高表达;而与H69、H1688细胞相比,H146、H446细胞中HOXA13基因分别相对高表达;下调H146、H446细胞HOXA13基因表达后,细胞增殖受到抑制,细胞周期S期阻滞,细胞凋亡率和药物敏感性增强。结论下调HOXA13基因可抑制小细胞肺癌细胞增殖和促进细胞凋亡,并增强细胞对化疗药物(阿霉素、顺铂和足叶乙甙)的敏感性,提示HOXA13基因可能在小细胞肺癌发生发展及耐药性的产生过程中发挥重要作用。
[Abstract]:Objective to investigate the expression of homologous box gene A13(HOXA13 gene in small cell lung cancer cells and its effects on proliferation, cell cycle, apoptosis and drug resistance of H146 and H446 cells before and after down-regulation. Methods the expression of HOXA13mRNA and protein in H69-H69AR-H168H146H446 and 16HBE of human bronchial epithelial cells was detected by real-time fluorescence quantitative PCR and Western blot assay, and the expression of HOXA13 gene in H146 and H446 cells was down-regulated by RNAi technique. The proliferation, cycle, apoptosis and drug resistance of HOXA13 gene cells were detected by CCK8 and flow cytometry. Results compared with 16HBE cells, the expression of HOXA13 gene was higher in H69-H1688-H146H446 cells, while that in H69-H1688 cells was higher than that in H69-H1688 cells, and the cell proliferation was inhibited and the cell cycle S phase was blocked after down-regulation of HOXA13 gene expression in H146H446 cells. Apoptosis rate and drug sensitivity were increased. Conclusion down-regulation of HOXA13 gene can inhibit proliferation and promote apoptosis of small cell lung cancer cells and enhance the sensitivity of cells to chemotherapeutic drugs (adriamycin, cisplatin and etoposide). The results suggest that HOXA13 gene may play an important role in the development of small cell lung cancer and the development of drug resistance.
【作者单位】：广东医科大学人体解剖学教研室;广东医科大学病理学系;南方医科大学珠江医院病理科;
【基金】：国家自然科学基金青年基金项目(No.81501242) 广东省自然科学基金项目(No.2017A030313644) 广东省医学科研基金项目(No.A2017330) 广东医科大学博士启动项目(No.B2017006)
【分类号】：R734.2

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