佐太和HgS对药物代谢酶和转运体基因表达的影响

发布时间：2018-05-21 02:09

  本文选题：佐太 + HgS　； 参考：《中国中药杂志》2017年21期

【摘要】：藏药佐太(主要含β-HgS)和中药朱砂(96%HgS)是传统医药中的配伍成分,其含重金属(汞)的安全问题和中西药合用时的相互作用问题日益受到关注。本实验就其对药物代谢基因的影响进行了研究。小鼠连续7 d口服给予不同剂量佐太、HgS(10,30,100,300 mg·kg-1),或氯化汞(Hg Cl2,33.6 mg·kg-1),RT-PCR检测药物代谢酶和转运体蛋白相关基因表达。佐太和HgS对Ⅰ相(CYP1A2,CYP2B10,CYP3A11,CYP4A10)和Ⅱ相(UGT1A1,UGT2A3,SULT1A1,SULT2A1)药物代谢酶以及转运体蛋白(OATP1A1,OATP1A4,OATP1B2,OATP2B1,MRP1,MRP2,MRP3,MRP4)均无明显影响,而Hg Cl2显著上调CYP2B10,CYP4A10,UGT1A1,UGT2A3,SULT1A1,SULT2A1,OATP1A4,MRP1,MRP3,MRP4,对OATP1A1有抑制作用。综上,佐太和HgS在300 mg·kg-1的剂量下对肝脏代谢基因的表达没有产生明显的影响,而1/10等汞量的Hg Cl2则明显上调或改变Ⅰ相、Ⅱ相药物代谢基因和转运体基因的表达。
[Abstract]:Zo Tai (mainly containing 尾 -HgS) and Chinese medicine cinnabar 96 HgS are the compatible components of traditional medicine. The safety of heavy metals (mercury) and the interaction between Chinese and western medicine have been paid more and more attention. In this study, the effect of drug metabolism gene was studied. Mice were administrated orally for 7 days with different doses of HgSX 1030100300mg kg-1C or mercuric chloride Cl2C2C2C33.6 mg kg-1a RT-PCR to detect the expression of drug metabolizing enzymes and transporter protein-related genes. 浣愬お鍜孒gS瀵光厾鐩，

本文编号：1917261