大白母猪LIF基因多态性及其对繁殖性状的影响

发布时间：2018-05-21 06:30

本文选题：大白猪 + 白血病抑制因子　；参考：《云南农业大学学报(自然科学)》2017年04期

【摘要】：【目的】为揭示白血病抑制因子(leukemia inhibitory factor,LIF)基因变异位点与母猪繁殖性状的关联。【方法】采用PCR产物直接测序法检测了405头大白母猪LIF基因C4950G和C6988T位点的多态性,结合1 716窝母猪繁殖性能记录,分析了单个位点基因型及两位点合并基因型对5个繁殖性状的影响。【结果】大白猪C4950G和C6988T位点分别以等位基因G和T的频率最高,且均处于Hardy-Weinberg平衡状态,杂合度较高。C4950G位点GG基因型母猪的总产仔数、产活仔数、仔猪初生窝重及21日龄成活数显著高于其余两种基因型(P0.05或P0.01),C6988T位点CT基因型母猪上述4个性状显著高于TT基因型(P0.05)。两个位点对母猪繁殖性能的影响呈现了明显的协同作用,合并基因型为GGCT的母猪,可较其他基因型显著提高总产仔数0.90~1.05头(P0.05或P0.01)、产活仔数0.54~0.88头(P0.05或P0.01)、仔猪21日龄成活数1.02~1.95头(P0.05或P0.01),仔猪初生窝重与21日龄窝重也更大(P0.05或P0.01)。【结论】进一步证实LIF基因与母猪繁殖性状间存在显著关联,但在不同品种(猪群)中,该基因的具体效应可能会有所不同。
[Abstract]:[objective] to investigate the association between the mutation site of leukemia inhibitory factor (LF) gene and reproductive traits in sows. [methods] the polymorphism of LIF gene C4950G and C6988T in 405 white sows was detected by direct sequencing of PCR products. Combined with the reproductive performance records of 1,716 litter sows, the effects of single locus genotype and two locus combined genotype on five reproductive traits were analyzed. [results] the frequencies of alleles G and T were the highest in large white pigs C4950G and C6988T, respectively. The total litter size and live litter size of GG genotype sows with high heterozygosity. The litter weight and survival number of 21 day old piglets were significantly higher than those of the other two genotypes (P0.05 or P0.01C6988T). The above four traits of CT genotypes were significantly higher than those of TT genotypes (P0.05). The effect of the two loci on the reproductive performance of sows showed obvious synergistic effect, which combined with GGCT. Compared with other genotypes, the total litter size was significantly increased by 0.90g / 1.05 or P0.01U, the number of live litter was 0.54 / 0.88 (P0.05 or P0.01A), the survival number of piglets was 1.02dw (P0.05) or P0.01g (P0.01g), and the litter weight of newborn piglets and 21-day-old litter weight was higher (P0.05 or P0.01g). [conclusion] LIF was further confirmed by P0.01G or P0.01G. [conclusion] the results showed that the litter weight of piglets was significantly higher than that of other genotypes (P 0.05 or P 0.01). [conclusion] There was a significant correlation between gene and reproductive traits in sows. But the specific effects of the gene may vary from breed to breed.
【作者单位】：云南农业大学动物科学技术学院;云南省德宏州芒市农业局畜牧站;云南惠嘉育种有限公司;
【基金】：NSFC-云南联合基金项目(U1402266) 云南省高校科技创新团队支持计划(云教科[2014]22号) 云南省现代农业生猪产业技术体系建设项目(2017KJTX0013)
【分类号】：S828

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